Anti-Mitofusin-1 Antibody (CAB9880)
- SKU:
- CAB9880
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Mitofusin-1 Antibody |
| Antibody SKU: | CAB9880 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 622-741 of human MFN1 (NP_284941.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, LO2, SW480, Mouse liver, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 622-741 of human MFN1 (NP_284941.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YGAL YLYE RLSW TTHA KERA FKQQ FVNY ATEK LRMI VSST SANC SHQV KQQI ATTF ARLC QQVD ITQK QLEE EIAR LPKE IDQL EKIQ NNSK LLRN KAVQ LENE LENF TKQF LPSS NEES |
| Gene ID: | 55669 |
| Uniprot: | Q8IWA4 |
| Cellular Location: | Cytoplasm, Mitochondrion outer membrane, Multi-pass membrane protein |
| Calculated MW: | 41kDa/71kDa/84kDa |
| Observed MW: | 84kDa |
| Synonyms: | MFN1, hfzo1, hfzo2 |
| Background: | The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. |
| UniProt Protein Function: | MFN1: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. Belongs to the mitofusin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; EC 3.6.5.-; Mitochondrial; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: 3q26.33 Cellular Component: mitochondrial outer membrane; mitochondrion Molecular Function:protein binding Biological Process: macroautophagy; mitochondrial fusion |
| NCBI Summary: | The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8IWA4 |
| NCBI GenInfo Identifier: | 150421594 |
| NCBI Gene ID: | 55669 |
| NCBI Accession: | Q8IWA4.2 |
| UniProt Secondary Accession: | Q8IWA4,O15323, O60639, Q9BZB5, Q9NWQ2, B2RAR1, D3DNR6 |
| UniProt Related Accession: | Q8IWA4 |
| Molecular Weight: | 71,401 Da |
| NCBI Full Name: | Mitofusin-1 |
| NCBI Synonym Full Names: | mitofusin 1 |
| NCBI Official Symbol: | MFN1 |
| NCBI Official Synonym Symbols: | hfzo1; hfzo2 |
| NCBI Protein Information: | mitofusin-1 |
| UniProt Protein Name: | Mitofusin-1 |
| UniProt Synonym Protein Names: | Fzo homolog; Transmembrane GTPase MFN1 |
| Protein Family: | Mitofusin |
| UniProt Gene Name: | MFN1 |
| UniProt Entry Name: | MFN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MFN1 antibody (CAB9880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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