Anti-METTL3 Antibody [KO Validated] (CAB19079)

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SKU:
CAB19079
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-METTL3 Antibody [KO Validated]
Antibody SKU:CAB19079
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human METTL3
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse brain, Mouse testis, 293T
Immunogen:A synthesized peptide derived from human METTL3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:56339
Uniprot:Q86U44
Cellular Location:Nucleus speckle
Calculated MW:70kDa
Observed MW:80kDa
Anti-METTL3 Antibody [KO Validated] (CAB19079)Western blot analysis of extracts from normal (control) and METTL3 knockout (KO) 293T cells, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-METTL3 Antibody [KO Validated] (CAB19079)Western blot analysis of extracts of various cell lines, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-METTL3 Antibody [KO Validated] (CAB19079)Immunofluorescence analysis of HeLa cells using METTL3 Rabbit mAb (CAB19079) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synonyms:IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3
Background:This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
UniProt Protein Function:METTL3: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. Belongs to the MT-A70-like family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.62; RNA processing

Chromosomal Location of Human Ortholog: 14q11.1

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity; RNA binding

Biological Process: circadian rhythm; stem cell maintenance; RNA methylation; gene expression; mRNA processing

NCBI Summary:This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
UniProt Code:Q86U44
NCBI GenInfo Identifier:21361827
NCBI Gene ID:56339
NCBI Accession:NP_062826.2
UniProt Secondary Accession:Q86U44,O14736, Q86V05, Q9HB32,
UniProt Related Accession:Q86U44
Molecular Weight:25,479 Da
NCBI Full Name:N6-adenosine-methyltransferase 70 kDa subunit
NCBI Synonym Full Names:methyltransferase like 3
NCBI Official Symbol:METTL3  
NCBI Official Synonym Symbols:M6A; IME4; Spo8; MT-A70  
NCBI Protein Information:N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase
UniProt Protein Name:N6-adenosine-methyltransferase 70 kDa subunit
UniProt Synonym Protein Names:Methyltransferase-like protein 3
Protein Family:N6-adenosine-methyltransferase
UniProt Gene Name:METTL3  
UniProt Entry Name:MTA70_HUMAN

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