Anti-METTL14 Antibody (CAB8530)
- SKU:
- CAB8530
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-METTL14 Antibody |
| Antibody SKU: | CAB8530 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, Raji, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human METTL14 (NP_066012.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDSR LQEI RERQ KLRR QLLA QQLG AESA DSIG AVLN SKDE QREI AETR ETCR ASYD TSAP NAKR KYLD EGET DEDK MEEY KDEL EMQQ DEEN LPYE EEIY KDSS TFLK GTQS LNPH NDYC QHFV DTGH RPQN FIRD VGLA DRFE EYPK LREL IRLK DELI AKSN TPPM YLQA DIEA FDIR ELTP KFDV ILLE PPLE EYYR ETGI TANE KC |
| Gene ID: | 57721 |
| Uniprot: | Q9HCE5 |
| Cellular Location: | Nucleus |
| Calculated MW: | 52kDa |
| Observed MW: | 60KDa |
| Synonyms: | METTL14, hMETTL14 |
| Background: |
| UniProt Protein Function: | The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27373337, PubMed:27281194). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27627798, PubMed:27373337, PubMed:27281194). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. |
| UniProt Code: | Q9HCE5 |
| NCBI GenInfo Identifier: | 24308265 |
| NCBI Gene ID: | 57721 |
| NCBI Accession: | NP_066012.1 |
| UniProt Secondary Accession: | Q9HCE5,Q969V2, A6NIG1, |
| UniProt Related Accession: | Q9HCE5 |
| Molecular Weight: | 52150 |
| NCBI Full Name: | N6-adenosine-methyltransferase non-catalytic subunit |
| NCBI Synonym Full Names: | methyltransferase like 14 |
| NCBI Official Symbol: | METTL14 |
| NCBI Official Synonym Symbols: | hMETTL14 |
| NCBI Protein Information: | N6-adenosine-methyltransferase non-catalytic subunit; N6-adenosine-methyltransferase subunit METTL14 |
| UniProt Protein Name: | N6-adenosine-methyltransferase non-catalytic subunit |
| UniProt Synonym Protein Names: | Methyltransferase-like protein 14 |
| Protein Family: | N6-adenosine-methyltransferase |
| UniProt Gene Name: | METTL14 |
 | Western blot analysis of extracts of various cell lines, using METTL14 antibody (CAB8530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of extracts of various cell lines, using METTL14 antibody (CAB8530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded Mouse brain using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat spleen using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human mammary cancer using METTL14 antibody (CAB8530) at dilution of 1:100 (40x lens). |
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