Anti-MDH2 Antibody (CAB19906)[KO Validated]

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SKU:
CAB19906
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-MDH2 Antibody [KO Validated]
Antibody SKU:CAB19906
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human MDH2.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant protein of human MDH2.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4191
Uniprot:P40926
Cellular Location:Mitochondrion matrix
Calculated MW:30kDa/35kDa
Observed MW:37KDa
Anti-MDH2 Antibody [KO Validated] (CAB19906)Western blot analysis of extracts from normal (control) and MDH2 knockout (KO) 293T cells, using MDH2 antibody (CAB19906) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Synonyms:MDH2, EIEE51, M-MDH, MDH, MGC:3559, MOR1
Background:Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MDH2: mitochondrial malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. May play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 1.1.1.37; Oxidoreductase

Chromosomal Location of Human Ortholog: 7cen-q22

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; plasma membrane; nucleus

Molecular Function:protein self-association; L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity

Biological Process: cellular metabolic process; malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pathogenesis; cellular carbohydrate metabolic process; internal protein amino acid acetylation; gluconeogenesis

NCBI Summary:Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
UniProt Code:P40926
NCBI GenInfo Identifier:215274114
NCBI Gene ID:4191
NCBI Accession:P40926.3
UniProt Secondary Accession:P40926,O43682, A8K414, B2RE78, B4DE44, E9PDB2,
UniProt Related Accession:P40926
Molecular Weight:338
NCBI Full Name:Malate dehydrogenase, mitochondrial
NCBI Synonym Full Names:malate dehydrogenase 2, NAD (mitochondrial)
NCBI Official Symbol:MDH2  
NCBI Official Synonym Symbols:MDH; MOR1; M-MDH; MGC:3559  
NCBI Protein Information:malate dehydrogenase, mitochondrial
UniProt Protein Name:Malate dehydrogenase, mitochondrial
Protein Family:Malate dehydrogenase
UniProt Gene Name:MDH2  
UniProt Entry Name:MDHM_HUMAN

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