Anti-MDC1 Antibody (CAB12714)
- SKU:
- CAB12714
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-MDC1 Antibody |
| Antibody SKU: | CAB12714 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MDC1 (NP_055456.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MDC1 (NP_055456.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEDT QAID WDVE EEEE TEQS SESL RCNV EPVG RLHI FSGA HGPE KDFP LHLG KNVV GRMP DCSV ALPF PSIS KQHA EIEI LAWD KAPI LRDC GSLN GTQI |
| Gene ID: | 9656 |
| Uniprot: | Q14676 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 116kDa/195kDa/197kDa/226kDa |
| Observed MW: | 226kDa |
| Synonyms: | MDC1, NFBD1 |
| Background: | The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. |
| UniProt Protein Function: | MDC1: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: chromosome; focal adhesion; nuclear body; nucleoplasm; nucleus Molecular Function:protein binding; protein C-terminus binding Biological Process: double-strand break repair via nonhomologous end joining; intra-S DNA damage checkpoint |
| NCBI Summary: | The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14676 |
| NCBI GenInfo Identifier: | 68565390 |
| NCBI Gene ID: | 9656 |
| NCBI Accession: | Q14676.3 |
| UniProt Secondary Accession: | Q14676,Q0EFC2, Q2L6H7, Q2TAZ4, Q5JP55, Q5JP56, Q5ST83 A2AB04, A2BF04, A2RRA8, A7YY86, B0S8A2, |
| UniProt Related Accession: | Q14676 |
| Molecular Weight: | 227kDa |
| NCBI Full Name: | Mediator of DNA damage checkpoint protein 1 |
| NCBI Synonym Full Names: | mediator of DNA damage checkpoint 1 |
| NCBI Official Symbol: | MDC1 |
| NCBI Official Synonym Symbols: | NFBD1 |
| NCBI Protein Information: | mediator of DNA damage checkpoint protein 1 |
| UniProt Protein Name: | Mediator of DNA damage checkpoint protein 1 |
| UniProt Synonym Protein Names: | Nuclear factor with BRCT domains 1 |
| Protein Family: | Mediator of DNA damage checkpoint protein |
| UniProt Gene Name: | MDC1 |
 | Western blot analysis of extracts of mouse testis, using MDC1 antibody (CAB12714) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using MDC1 Rabbit pAb (CAB12714) at dilution of 1:150 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using MDC1 Rabbit pAb (CAB12714) at dilution of 1:150 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using MDC1 Rabbit pAb (CAB12714) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining. |
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