Anti-MCM8 항체 (CAB12922)

Antibody Name:	Anti-MCM8 Antibody
Antibody SKU:	CAB12922
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 591-840 of human MCM8 (NP_115874.3).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	LO2, HeLa, A-431

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 591-840 of human MCM8 (NP_115874.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FILL DTPN EHHD HLLS EHVI AIRA GKQR TISS ATVA RMNS QDSN TSVL EVVS EKPL SERL KVVP GETI DPIP HQLL RKYI GYAR QYVY PRLS TEAA RVLQ DFYL ELRK QSQR LNSS PITT RQLE SLIR LTEA RARL ELRE EATK EDAE DIVE IMKY SMLG TYSD EFGN LDFE RSQH GSGM SNRS TAKR FISA LNNV AERT YNNI FQFH QLRQ IAKE LNIQ VADF ENFI GSLN DQGY LLKK GPKV YQLQ TM
Gene ID:	84515
Uniprot:	Q9UJA3
Cellular Location:	Nucleus
Calculated MW:	88kDa/91kDa/93kDa/97kDa
Observed MW:	110kDa

Synonyms:

MCM8, C20orf154, POF10, dJ967N21.5

Background:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	MCM8: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle. Belongs to the MCM family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 20p12.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; helicase activity; ATP binding Biological Process: male gamete generation; female gamete generation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; response to DNA damage stimulus; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle Disease: Premature Ovarian Failure 10
NCBI Summary:	The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]
UniProt Code:	Q9UJA3
NCBI GenInfo Identifier:	27805609
NCBI Gene ID:	84515
NCBI Accession:	Q9UJA3.2
UniProt Secondary Accession:	Q9UJA3,Q495R4, Q495R6, Q495R7, Q86US4, Q969I5, B2RBG7 D3DW08, E7EQU7,
UniProt Related Accession:	Q9UJA3
Molecular Weight:	840
NCBI Full Name:	DNA helicase MCM8
NCBI Synonym Full Names:	minichromosome maintenance complex component 8
NCBI Official Symbol:	MCM8
NCBI Official Synonym Symbols:	C20orf154; dJ967N21.5
NCBI Protein Information:	DNA helicase MCM8; REC homolog; DNA replication licensing factor MCM8; MCM8 minichromosome maintenance deficient 8
UniProt Protein Name:	DNA helicase MCM8
UniProt Synonym Protein Names:	Minichromosome maintenance 8
Protein Family:	DNA helicase
UniProt Gene Name:	MCM8
UniProt Entry Name:	MCM8_HUMAN

Western blot analysis of extracts of various cell lines, using MCM8 antibody (CAB12922) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

MCM8 Antibody (PACO10425)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)