Anti-MCM5 Antibody (CAB5556)
- SKU:
- CAB5556
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-MCM5 Antibody |
| Antibody SKU: | CAB5556 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:1000 - 1:3000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, SW620, Mouse spleen, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGF DDPG IFYS DSFG GDAQ ADEG QARK SQLQ RRFK EFLR QYRV GTDR TGFT FKYR DELK RHYN LGEY WIEV EMED LASF DEDL ADYL YKQP AEHL QLLE EAAK EVAD EVTR PRPS GEEV LQDI QVML KSDA SPSS IRSL KSDM MSHL VKIP GIII AASA VRAK ATRI SIQC RSCR NTLT NIAM RPGL EGYA LPRK CNTD QAGR PKCP LDPY FIMP DKCK CVDF QTLK LQ |
| Gene ID: | 4174 |
| Uniprot: | P33992 |
| Cellular Location: | Nucleus |
| Calculated MW: | 82kDa |
| Observed MW: | 100kDa |
| Synonyms: | MCM5, CDC46, P1-CDC46 |
| Background: | The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. |
| UniProt Protein Function: | MCM5: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP. Belongs to the MCM family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; MCM complex; membrane; nucleus Molecular Function:DNA helicase activity; protein binding; DNA binding; ATP binding Biological Process: DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P33992 |
| NCBI GenInfo Identifier: | 19858646 |
| NCBI Gene ID: | 4174 |
| NCBI Accession: | P33992.5 |
| UniProt Secondary Accession: | P33992,O60785, Q14578, Q9BTJ4, Q9BWL8, |
| UniProt Related Accession: | P33992 |
| Molecular Weight: | 82,286 Da |
| NCBI Full Name: | DNA replication licensing factor MCM5 |
| NCBI Synonym Full Names: | minichromosome maintenance complex component 5 |
| NCBI Official Symbol: | MCM5 |
| NCBI Official Synonym Symbols: | CDC46; P1-CDC46 |
| NCBI Protein Information: | DNA replication licensing factor MCM5; CDC46 homolog; minichromosome maintenance deficient 5 (cell division cycle 46); MCM5 minichromosome maintenance deficient 5, cell division cycle 46 |
| UniProt Protein Name: | DNA replication licensing factor MCM5 |
| UniProt Synonym Protein Names: | CDC46 homolog; P1-CDC46 |
| Protein Family: | Minichromosome maintenance protein |
| UniProt Gene Name: | MCM5 |
| UniProt Entry Name: | MCM5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCM5 antibody (CAB5556) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit pAb (CAB5556) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug MCM5 antibody (CAB5556). Western blot was performed from the immunoprecipitate using MCM5 antibody (CAB5556) at a dilition of 1:1000. |
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