Anti-MCL1 Antibody (CAB0250)
- SKU:
- CAB0250
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-MCL1 Antibody |
| Antibody SKU: | CAB0250 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 120-220 of human MCL1 (NP_068779.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, Raji, Mouse lung, Mouse thymus |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 120-220 of human MCL1 (NP_068779.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSPE EELD GYEP EPLG KRPA VLPL LELV GESG NNTS TDGS LPST PPPA EEEE DELY RQSL EIIS RYLR EQAT GAKD TKPM GRSG ATSR KALE TLRR VGDG V |
| Gene ID: | 4170 |
| Uniprot: | Q07820 |
| Cellular Location: | Cytoplasm, Membrane, Mitochondrion, Nucleus, Single-pass membrane protein, nucleoplasm |
| Calculated MW: | 28kDa/37kDa |
| Observed MW: | 37kDa |
| Synonyms: | MCL1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT, MCL1 |
| Background: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. |
| UniProt Protein Function: | MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing. |
| UniProt Protein Details: | Protein type:Inhibitor; Apoptosis; Mitochondrial; Channel, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21 Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination |
| NCBI Summary: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010] |
| UniProt Code: | Q07820 |
| NCBI GenInfo Identifier: | 83304396 |
| NCBI Gene ID: | 4170 |
| NCBI Accession: | Q07820.3 |
| UniProt Secondary Accession: | Q07820,Q9HD91, Q9NRQ3, Q9NRQ4, Q9UHR7, Q9UHR8, Q9UHR9 Q9UNJ1, B2R6B2, D3DV03, D3DV04, |
| UniProt Related Accession: | Q07820 |
| Molecular Weight: | 350 |
| NCBI Full Name: | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| NCBI Synonym Full Names: | myeloid cell leukemia 1 |
| NCBI Official Symbol: | MCL1 |
| NCBI Official Synonym Symbols: | TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT |
| NCBI Protein Information: | induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; myeloid cell leukemia ES; bcl-2-related protein EAT/mcl1; myeloid cell leukemia sequence 1 (BCL2-related) |
| UniProt Protein Name: | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| UniProt Synonym Protein Names: | Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT |
| Protein Family: | Minichromosome loss protein |
| UniProt Gene Name: | MCL1 |
| UniProt Entry Name: | MCL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCL1 antibody (CAB0250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using MCL1 antibody (CAB0250) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using MCL1 antibody (CAB0250) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using MCL1 antibody (CAB0250) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A431 cells using MCL1 antibody (CAB0250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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