Anti-MARK2 Antibody (CAB16706)
- SKU:
- CAB16706
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MARK2 Antibody |
| Antibody SKU: | CAB16706 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human MARK2 |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Raji, BxPC-3, BT-474, mouse testis, mouse heart, rat testis |
| Immunogen: | Recombinant protein of human MARK2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2011 |
| Uniprot: | Q7KZI7 |
| Cellular Location: | Cell membrane, Cytoplasm, Lateral cell membrane, Peripheral membrane protein, cytoskeleton |
| Calculated MW: | 77-87kDa |
| Observed MW: | 78kDa |
| Synonyms: | MARK2, EMK-1, EMK1, PAR-1, Par-1b, Par1b |
| Background: | This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MARK2: a CAMK protein kinase of the CAMKL group. Plays a role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. 12 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.26; CAMK group; CAMKL family; MARK subfamily Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: membrane; mitochondrion; plasma membrane; actin filament; basal cortex; nucleus; lateral plasma membrane Molecular Function:protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; magnesium ion binding; protein kinase activator activity; lipid binding; ATP binding Biological Process: establishment and/or maintenance of epithelial cell polarity; Wnt receptor signaling pathway; establishment of cell polarity; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitochondrion degradation; neuron migration; activation of protein kinase activity; mitochondrion localization; regulation of axonogenesis; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis |
| NCBI Summary: | This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009] |
| UniProt Code: | Q7KZI7 |
| NCBI GenInfo Identifier: | 62510922 |
| NCBI Gene ID: | 2011 |
| NCBI Accession: | Q7KZI7.2 |
| UniProt Secondary Accession: | Q7KZI7,Q15449, Q15524, Q5XGA3, Q68A18, Q96HB3, Q96RG0 |
| UniProt Related Accession: | Q7KZI7 |
| Molecular Weight: | 788 |
| NCBI Full Name: | Serine/threonine-protein kinase MARK2 |
| NCBI Synonym Full Names: | MAP/microtubule affinity-regulating kinase 2 |
| NCBI Official Symbol: | MARK2 |
| NCBI Official Synonym Symbols: | EMK1; EMK-1; PAR-1; Par1b; Par-1b |
| NCBI Protein Information: | serine/threonine-protein kinase MARK2; PAR1 homolog b; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK |
| UniProt Protein Name: | Serine/threonine-protein kinase MARK2 |
| UniProt Synonym Protein Names: | ELKL motif kinase 1; EMK-1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b; Par-1b; Par1b |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | MARK2 |
| UniProt Entry Name: | MARK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MARK2 antibody (CAB16706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using MARK2 antibody (CAB16706) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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