Anti-MAP3K4 Antibody (CAB16918)
- SKU:
- CAB16918
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| Antibody Name: | Anti-MAP3K4 Antibody |
| Antibody SKU: | CAB16918 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | K-562, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K4 (NP_005913.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MREA AAAL VPPP AFAV TPAA AMEE PPPP PPPP PPPP EPET ESEP ECCL AARQ EGTL GDSA CKSP ESDL EDFS DETN TENL YGTS PPST PRQM KRMS TKHQ |
| Gene ID: | 4216 |
| Uniprot: | Q9Y6R4 |
| Cellular Location: | |
| Calculated MW: | 177kDa/181kDa |
| Observed MW: | 170kDa |
 | Western blot - MAP3K4 Rabbit pAb (CAB16918) |
| Synonyms: | MAP3K4, MAPKKK4, MEKK 4, MEKK4, MTK1, PRO0412 |
| Background: | The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. |
| UniProt Protein Function: | MEKK4: a protein kinase of the STE11 family. A mediator of environmental stresses. Phosphorylates and activates MKK4, -6, and-7, which in turn activate the p38 AND JNK MAPK pathways. |
| UniProt Protein Details: | Protein type:Protein kinase, STE; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE11 family Chromosomal Location of Human Ortholog: 6q26 Cellular Component: perinuclear region of cytoplasm; cytoplasm Molecular Function:MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding Biological Process: positive regulation of JNK activity; activation of MAPKK activity; regulation of gene expression; activation of MAPK activity; MAPKKK cascade; protein amino acid phosphorylation; response to UV-C; placenta development; male germ-line sex determination |
| NCBI Summary: | The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014] |
| UniProt Code: | Q9Y6R4 |
| NCBI GenInfo Identifier: | 296434576 |
| NCBI Gene ID: | 4216 |
| NCBI Accession: | Q9Y6R4.2 |
| UniProt Secondary Accession: | Q9Y6R4,Q5VTT8, Q5VTT9, Q92612, Q9H408, A6H8W0, B7ZLD3 B9EG75, |
| UniProt Related Accession: | Q9Y6R4 |
| Molecular Weight: | 177,014 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 4 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 4 |
| NCBI Official Symbol: | MAP3K4 |
| NCBI Official Synonym Symbols: | MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412 |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 4; MEK kinase 4; MAP three kinase 1; MAP/ERK kinase kinase 4; MAPK/ERK kinase kinase 4; predicted protein of HQ0412; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of; dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 4 |
| UniProt Synonym Protein Names: | MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4 |
| Protein Family: | Mitogen-activated protein kinase kinase kinase |
| UniProt Gene Name: | MAP3K4 |
| UniProt Entry Name: | M3K4_HUMAN |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
