Anti-MAP1B 항체 (CAB3305)

Antibody Name:	Anti-MAP1B Antibody
Antibody SKU:	CAB3305
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, 293T, HT-1080, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1820-2100 of human MAP1B (NP_005900.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PPID AASA EPYG FRAS VLFD TMQH HLAL NRDL STPG LEKD SGGK TPGD FSYA YQKP EETT RSPD EEDY DYES YEKT TRTS DVGG YYYE KIER TTKS PSDS GYSY ETIG KTTK TPED GDYS YEII EKTT RTPE EGGY SYDI SEKT TSPP EVSG YSYE KTER SRRL LDDI SNGY DDSE DGGH TLGD PSYS YETT EKIT SFPE SEGY SYET STKT TRTP DTST YCYE TAEK ITRT PQAS TYSY ETSD LCYT AEKK SPSE ARQD VDLC LVSS CEYK HPKT ELSP S
Gene ID:	4131
Uniprot:	P46821
Cellular Location:	Cell junction, Cell projection, Cytoplasm, cytoskeleton, dendritic spine, synapse
Calculated MW:	270kDa
Observed MW:	350kDa

Synonyms:

MAP1B, FUTSCH, MAP5, PPP1R102

Background:

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.

UniProt Protein Function:	MAP1B: the function of brain MAPS is essentially unknown. Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B Binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 5q13 Cellular Component: microtubule; microtubule associated complex; postsynaptic density; cytoplasm; dendritic spine; plasma membrane; cell junction; cytosol Molecular Function:protein binding; microtubule binding; structural molecule activity Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of axon extension; dendrite development; cellular process; microtubule bundle formation; mitochondrion transport along microtubule
NCBI Summary:	This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system. [provided by RefSeq, Jul 2008]
UniProt Code:	P46821
NCBI GenInfo Identifier:	317373388
NCBI Gene ID:	4131
NCBI Accession:	P46821.2
UniProt Secondary Accession:	P46821,P14873, P15205,
UniProt Related Accession:	P46821
Molecular Weight:	2468
NCBI Full Name:	Microtubule-associated protein 1B
NCBI Synonym Full Names:	microtubule-associated protein 1B
NCBI Official Symbol:	MAP1B
NCBI Official Synonym Symbols:	MAP5; FUTSCH; PPP1R102
NCBI Protein Information:	microtubule-associated protein 1B; MAP-1B; protein phosphatase 1, regulatory subunit 102
UniProt Protein Name:	Microtubule-associated protein 1B
Protein Family:	Methionine aminopeptidase
UniProt Gene Name:	MAP1B
UniProt Entry Name:	MAP1B_HUMAN

	Western blot analysis of extracts of various cell lines, using MAP1B antibody (CAB3305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using MAP1B antibody (CAB3305) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using MAP1B antibody (CAB3305) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using MAP1B antibody (CAB3305) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MAP1B Antibody (PACO10358)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)