Anti-MANF Antibody (CAB19923)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19923
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-MANF Antibody [KO Validated]
Antibody SKU:CAB19923
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human MANF.
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human MANF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:7873
Uniprot:P55145
Cellular Location:Secreted
Calculated MW:20kDa
Observed MW:16KDa
Anti-MANF Antibody [KO Validated] (CAB19923)Western blot analysis of extracts from normal (control) and MANF knockout (KO) 293T cells, using MANF antibody (CAB19923) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30S.
Synonyms:MANF, ARMET, ARP
Background:The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.
UniProt Protein Function:ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleus

Disease: Pancreatic Cancer

NCBI Summary:The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
UniProt Code:P55145
NCBI GenInfo Identifier:332278201
NCBI Gene ID:7873
NCBI Accession:P55145.3
UniProt Secondary Accession:P55145,Q14CX4, Q86U67, Q96IS4,
UniProt Related Accession:P55145
Molecular Weight:20,700 Da
NCBI Full Name:Mesencephalic astrocyte-derived neurotrophic factor
NCBI Synonym Full Names:mesencephalic astrocyte derived neurotrophic factor
NCBI Official Symbol:MANF  
NCBI Official Synonym Symbols:ARP; ARMET  
NCBI Protein Information:mesencephalic astrocyte-derived neurotrophic factor
UniProt Protein Name:Mesencephalic astrocyte-derived neurotrophic factor
UniProt Synonym Protein Names:Arginine-rich protein; Protein ARMET
Protein Family:MANF/CDNF-like protein
UniProt Gene Name:MANF  
UniProt Entry Name:MANF_HUMAN

0 Reviews

View AllClose

Related Products