Anti-MAN2A1 Antibody (CAB10227)
- SKU:
- CAB10227
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MAN2A1 Antibody |
| Antibody SKU: | CAB10227 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 865-1144 of human MAN2A1 (NP_002363.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, MCF7, NCI-H460, HT-29, HT-1080, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 865-1144 of human MAN2A1 (NP_002363.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SNIV DIRK VYNR EIAM KISS DIKS QNRF YTDL NGYQ IQPR MTLS KLPL QANV YPMT TMAY IQDA KHRL TLLS AQSL GVSS LNSG QIEV IMDR RLMQ DDNR GLEQ GIQD NKIT ANLF RILL EKRS AVNT EEEK KSVS YPSL LSHI TSSL MNHP VIPM ANKF SSPT LELQ GEFS PLQS SLPC DIHL VNLR TIQS KVGN GHSN EAAL ILHR KGFD CRFS SKGT GLFC STTQ GKIL VQKL LNKF IVES LTPS SLSL MHSP PGTQ NISE INLS PMEI STFR IQLR |
| Gene ID: | 4124 |
| Uniprot: | Q16706 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 131kDa |
| Observed MW: | 131-170kDa |
| Synonyms: | MAN2A1, AMan II, GOLIM7, MANA2, MANII, AManII |
| Background: | This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. |
| UniProt Protein Function: | MAN2A1: Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. Belongs to the glycosyl hydrolase 38 family. |
| UniProt Protein Details: | Protein type:Hydrolase; Membrane protein, integral; EC 3.2.1.114; Glycan Metabolism - N-glycan biosynthesis Chromosomal Location of Human Ortholog: 5q21.3 Cellular Component: Golgi membrane; membrane Molecular Function:mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Biological Process: mannose metabolic process; N-glycan processing; protein deglycosylation |
| NCBI Summary: | This gene encodes a glycosyl hydrolase that localizes to the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq, Dec 2013] |
| UniProt Code: | Q16706 |
| NCBI GenInfo Identifier: | 146345453 |
| NCBI Gene ID: | 4124 |
| NCBI Accession: | Q16706.2 |
| UniProt Secondary Accession: | Q16706,Q16767, |
| UniProt Related Accession: | Q16706 |
| Molecular Weight: | 131,141 Da |
| NCBI Full Name: | Alpha-mannosidase 2 |
| NCBI Synonym Full Names: | mannosidase alpha class 2A member 1 |
| NCBI Official Symbol: | MAN2A1 |
| NCBI Official Synonym Symbols: | MANA2; MANII; GOLIM7; AMan II |
| NCBI Protein Information: | alpha-mannosidase 2 |
| UniProt Protein Name: | Alpha-mannosidase 2 |
| UniProt Synonym Protein Names: | Golgi alpha-mannosidase II; AMan II; Man II; Mannosidase alpha class 2A member 1; Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase |
| UniProt Gene Name: | MAN2A1 |
| UniProt Entry Name: | MA2A1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAN2A1 antibody (CAB10227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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