Anti-MAN1A2 Antibody (CAB14271)
- SKU:
- CAB14271
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-MAN1A2 Antibody |
| Antibody SKU: | CAB14271 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human MAN1A2 (NP_006690.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | LO2, A-549, Mouse brain, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-180 of human MAN1A2 (NP_006690.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SSKH KRFD LGLE DVLI PHVD AGKG AKNP GVFL IHGP DEHR HREE EERL RNKI RADH EKAL EEAK EKLR KSRE EIRA EIQT EKNK VVQE MKIK ENKP LPPV PIPN LVGI RGGD PEDN DIRE K |
| Gene ID: | 10905 |
| Uniprot: | O60476 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 73kDa |
| Observed MW: | 73kDa |
| Synonyms: | MAN1A2, MAN1B |
| Background: |
| UniProt Protein Function: | MAN1A2: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). Belongs to the glycosyl hydrolase 47 family. |
| UniProt Protein Details: | Protein type:Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; EC 3.2.1.113; Hydrolase Chromosomal Location of Human Ortholog: 1p13 Cellular Component: endoplasmic reticulum; Golgi apparatus; Golgi membrane; membrane Molecular Function:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Biological Process: N-glycan processing |
| NCBI Summary: | Alpha-mannosidases function at different stages of N-glycan maturation in mammalian cells. See MAN2A1 (MIM 154582) for general information.[supplied by OMIM, Mar 2008] |
| UniProt Code: | O60476 |
| NCBI GenInfo Identifier: | 17368019 |
| NCBI Gene ID: | 10905 |
| NCBI Accession: | O60476.1 |
| UniProt Secondary Accession: | O60476,Q9H510, |
| UniProt Related Accession: | O60476 |
| Molecular Weight: | 73,004 Da |
| NCBI Full Name: | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB |
| NCBI Synonym Full Names: | mannosidase alpha class 1A member 2 |
| NCBI Official Symbol: | MAN1A2 |
| NCBI Official Synonym Symbols: | MAN1B |
| NCBI Protein Information: | mannosyl-oligosaccharide 1,2-alpha-mannosidase IB |
| UniProt Protein Name: | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB |
| UniProt Synonym Protein Names: | Mannosidase alpha class 1A member 2; Processing alpha-1,2-mannosidase IB; Alpha-1,2-mannosidase IB |
| Protein Family: | Mannosyl-oligosaccharide 1,2-alpha-mannosidase |
| UniProt Gene Name: | MAN1A2 |
| UniProt Entry Name: | MA1A2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAN1A2 antibody (CAB14271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded Human colon carcinoma using MAN1A2 Rabbit pAb (CAB14271) at dilution of 1:100 (40x lens). |
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