Anti-MAD2L2 Antibody (CAB9861)
- SKU:
- CAB9861
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-MAD2L2 Antibody |
| Antibody SKU: | CAB9861 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2L2 (NP_006332.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-375, K-562, SW480, HeLa, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2L2 (NP_006332.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTTL TRQD LNFG QVVA DVLC EFLE VAVH LILY VREV YPVG IFQK RKKY NVPV QMSC HPEL NQYI QDTL HCVK PLLE KNDV EKVV VVIL DKEH RPVE KFVF EITQ PPLL SISS DSLL SHVE QLLR AFIL KISV CDAV LDHN PPGC TFTV LVHT REAA TRNM EKIQ VIKD FPWI LADE QDVH MHDP RLIP LKTM TSDI LKMQ LYVE ERAH KGS |
| Gene ID: | 10459 |
| Uniprot: | Q9UI95 |
| Cellular Location: | Cytoplasm, Nucleus, cytoskeleton, spindle |
| Calculated MW: | 24kDa |
| Observed MW: | 25kDa |
| Synonyms: | MAD2L2, FANCV, MAD2B, POLZ2, REV7 |
| Background: | The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. |
| UniProt Protein Function: | MAD2L2: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: anaphase-promoting complex; cytosol; nucleolus; nucleoplasm; nucleus; spindle; zeta DNA polymerase complex Molecular Function:JUN kinase binding; protein binding Biological Process: actin filament organization; DNA damage response, signal transduction resulting in transcription; double-strand break repair; error-prone postreplication DNA repair; mitotic cell cycle spindle assembly checkpoint; negative regulation of protein catabolic process; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription, DNA-dependent; regulation of cell growth Disease: Fanconi Anemia, Complementation Group V |
| NCBI Summary: | The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UI95 |
| NCBI GenInfo Identifier: | 12643889 |
| NCBI Gene ID: | 10459 |
| NCBI Accession: | Q9UI95.2 |
| UniProt Secondary Accession: | Q9UI95,Q5TGW7, Q9UNA7, Q9Y6I6, B3KNE3, |
| UniProt Related Accession: | Q9UI95 |
| Molecular Weight: | 24kDa |
| NCBI Full Name: | Mitotic spindle assembly checkpoint protein MAD2B |
| NCBI Synonym Full Names: | mitotic arrest deficient 2 like 2 |
| NCBI Official Symbol: | MAD2L2 |
| NCBI Official Synonym Symbols: | REV7; FANCV; MAD2B; POLZ2 |
| NCBI Protein Information: | mitotic spindle assembly checkpoint protein MAD2B |
| UniProt Protein Name: | Mitotic spindle assembly checkpoint protein MAD2B |
| UniProt Synonym Protein Names: | Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7 |
| Protein Family: | Mitotic spindle assembly checkpoint protein |
| UniProt Gene Name: | MAD2L2 |
 | Western blot analysis of extracts of various cell lines, using MAD2L2 antibody (CAB9861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using MAD2L2 antibody (CAB9861) at dilution of 1:100 (40x lens). |
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