Anti-MAD1L1 Antibody (CAB1153)
- SKU:
- CAB1153
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-MAD1L1 Antibody |
| Antibody SKU: | CAB1153 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, HepG2, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RGLE KARQ QLQE ELRQ VSGQ LLEE RKKR ETHE ALAR RLQK RVLL LTKE RDGM RAIL GSYD SELT PAEY SPQL TRRM REAE DMVQ KVHS HSAE MEAQ LSQA LEEL GGQK QRAD MLEM ELKM LKSQ SSSA EQSF LFSR EEAD TLRL KVEE LEGE RSRL EEEK RMLE AQLE RRAL QGDY DQSR TKVL HMSL NPTS VARQ RLRE DHSQ LQAE CERL RGLL RAME RGGT VPAD LEAA AASL PSSK EVAE LKKQ VESA ELKN QRLK EVFQ TKIQ EFRK ACYT LTGY QIDI TTEN QYRL TSLY AEHP GDCL IFKA TSPS GSKM QLLE TEFS HTVG ELIE VHLR RQDS I |
| Gene ID: | 8379 |
| Uniprot: | Q9Y6D9 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle |
| Calculated MW: | 72kDa/83kDa |
| Observed MW: | 95kDa |
| Synonyms: | MAD1L1, MAD1, PIG9, TP53I9, TXBP181 |
| Background: | MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Cytoskeletal Chromosomal Location of Human Ortholog: 7p22 Cellular Component: kinetochore; centrosome; cytoplasm; spindle; nuclear pore; nucleus; cytosol; actin cytoskeleton Molecular Function:protein binding Biological Process: mitosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle Disease: Prostate Cancer |
| NCBI Summary: | MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y6D9 |
| NCBI GenInfo Identifier: | 52783153 |
| NCBI Gene ID: | 8379 |
| NCBI Accession: | Q9Y6D9.2 |
| UniProt Secondary Accession: | Q9Y6D9,Q13312, Q75MI0, Q86UM4, Q9UNH0, B3KR41, |
| UniProt Related Accession: | Q9Y6D9 |
| Molecular Weight: | 718 |
| NCBI Full Name: | Mitotic spindle assembly checkpoint protein MAD1 |
| NCBI Synonym Full Names: | MAD1 mitotic arrest deficient-like 1 (yeast) |
| NCBI Official Symbol: | MAD1L1 |
| NCBI Official Synonym Symbols: | MAD1; PIG9; TP53I9; TXBP181 |
| NCBI Protein Information: | mitotic spindle assembly checkpoint protein MAD1; MAD1-like protein 1; tax-binding protein 181; tumor protein p53 inducible protein 9; mitotic checkpoint MAD1 protein homolog; mitotic arrest deficient 1-like protein 1; mitotic-arrest deficient 1, yeast, homolog-like 1 |
| UniProt Protein Name: | Mitotic spindle assembly checkpoint protein MAD1 |
| UniProt Synonym Protein Names: | Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181 |
| Protein Family: | Mitotic spindle assembly checkpoint protein |
| UniProt Gene Name: | MAD1L1 |
| UniProt Entry Name: | MD1L1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (CAB1153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (CAB1153) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (CAB1153) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (CAB1153). |
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