Anti-LPCAT4 항체 (CAB14316)

Antibody Name:	Anti-LPCAT4 Antibody
Antibody SKU:	CAB14316
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 345-524 of human LPCAT4 (NP_705841.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HT-29, HeLa, HepG2, Mouse brain, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 345-524 of human LPCAT4 (NP_705841.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGAE PGRS RMIS QEEF ARQL QLSD PQTV AGAF GYFQ QDTK GLVD FRDV ALAL AALD GGRS LEEL TRLA FELF AEEQ AEGP NRLL YKDG FSTI LHLL LGSP HPAA TALH AELC QAGS SQGL SLCQ FQNF SLHD PLYG KLFS TYLR PPHT SRGT SQTP NASS PGNP TALA NGTV QAPK QKGD
Gene ID:	254531
Uniprot:	Q643R3
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	57kDa
Observed MW:	57kDa

Synonyms:	LPCAT4, AGPAT7, AYTL3, LPAAT-eta, LPEAT2
Background:

UniProt Protein Function:	AGRN: Plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Ligand of the MUSK signaling complex that directly binds LRP4 in this complex and induces the phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Defects in AGRN are a cause of myasthenia, limb-girdle, familial (LGM). A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function.
UniProt Protein Details:	Protein type:Secreted; Cell adhesion; Extracellular matrix; Cell development/differentiation; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; cell surface; Golgi lumen; cytoplasm; integral to membrane; extracellular region; plasma membrane; synapse; basal lamina; cell junction Molecular Function:heparan sulfate proteoglycan binding; protein binding; structural constituent of cytoskeleton; acetylcholine receptor regulator activity; laminin binding; calcium ion binding; sialic acid binding Biological Process: positive regulation of filopodium formation; phototransduction, visible light; axon guidance; clustering of voltage-gated sodium channels; extracellular matrix organization and biogenesis; acetylcholine receptor signaling, muscarinic pathway; glycosaminoglycan metabolic process; neurotransmitter receptor metabolic process; pathogenesis; positive regulation of synaptic growth at neuromuscular junction; signal transduction; receptor clustering; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; plasma membrane organization and biogenesis; positive regulation of neuron apoptosis; carbohydrate metabolic process; synapse organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity; retinoid metabolic process; neuromuscular junction development Disease: Myasthenic Syndrome, Congenital, With Pre- And Postsynaptic Defects
NCBI Summary:	Members of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction (Ye et al., 2005 [PubMed 16243729]).[supplied by OMIM, May 2008]
UniProt Code:	Q643R3
NCBI GenInfo Identifier:	87116681
NCBI Gene ID:	254531
NCBI Accession:	NP_705841
UniProt Related Accession:	Q643R3
Molecular Weight:
NCBI Full Name:	lysophospholipid acyltransferase LPCAT4
NCBI Synonym Full Names:	lysophosphatidylcholine acyltransferase 4
NCBI Official Symbol:	LPCAT4
NCBI Official Synonym Symbols:	AYTL3; AGPAT7; LPEAT2; LPAAT-eta
NCBI Protein Information:	lysophospholipid acyltransferase LPCAT4
UniProt Protein Name:	Agrin
Protein Family:	Agrin
UniProt Gene Name:	AGRN
UniProt Entry Name:	AGRIN_HUMAN

Western blot analysis of extracts of various cell lines, using LPCAT4 antibody (CAB14316) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

LPCAT4 Antibody (PACO10281)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)