Anti-LMAN1 Antibody (CAB10440)
- SKU:
- CAB10440
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-LMAN1 Antibody |
| Antibody SKU: | CAB10440 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, LO2, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PGKE PPTP DKEI SEKE KEKY QEEF EHFQ QELD KKKE EFQK GHPD LQGQ PAEE IFES VGDR ELRQ VFEG QNRI HLEI KQLN RQLD MILD EQRR YVSS LTEE ISKR GAGM PGQH GQIT QQEL DTVV KTQH EILR QVNE MKNS MSET VRLV SGMQ HPGS AGGV YETT QHFI DIKE HLHI VKRD IDNL VQRN MPSN EKPK CPEL PPFP SCLS TVH |
| Gene ID: | 3998 |
| Uniprot: | P49257 |
| Cellular Location: | Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Single-pass membrane protein, Single-pass type I membrane protein |
| Calculated MW: | 57kDa |
| Observed MW: | 55kDa |
| Synonyms: | LMAN1, ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58 |
| Background: | The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. |
| UniProt Protein Function: | LMAN1: Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Defects in LMAN1 are THE cause of factor V and factor VIII combined deficiency type 1 (F5F8D1); also known as multiple coagulation factor deficiency I (MCFD1). F5F8D1 is an autosomal recessive blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 18q21.32 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment; ER-Golgi intermediate compartment membrane; membrane Molecular Function:protein binding; unfolded protein binding Biological Process: blood coagulation; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; protein amino acid N-linked glycosylation via asparagine; protein exit from endoplasmic reticulum; protein folding Disease: Factor V And Factor Viii, Combined Deficiency Of, 1 |
| NCBI Summary: | The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015] |
| UniProt Code: | P49257 |
| NCBI GenInfo Identifier: | 22261801 |
| NCBI Gene ID: | 3998 |
| NCBI Accession: | P49257.2 |
| UniProt Secondary Accession: | P49257,Q12895, Q8N5I7, Q9UQG1, Q9UQG2, Q9UQG3, Q9UQG4 Q9UQG5, Q9UQG6, Q9UQG7, Q9UQG8, Q9UQG9, |
| UniProt Related Accession: | P49257 |
| Molecular Weight: | 58kDa |
| NCBI Full Name: | Protein ERGIC-53 |
| NCBI Synonym Full Names: | lectin, mannose binding 1 |
| NCBI Official Symbol: | LMAN1 |
| NCBI Official Synonym Symbols: | MR60; gp58; F5F8D; FMFD1; MCFD1; ERGIC53; ERGIC-53 |
| NCBI Protein Information: | protein ERGIC-53 |
| UniProt Protein Name: | Protein ERGIC-53 |
| UniProt Synonym Protein Names: | ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose-binding 1 |
| Protein Family: | Protein |
| UniProt Gene Name: | LMAN1 |
 | Western blot analysis of extracts of various cell lines, using LMAN1 antibody (CAB10440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using LMAN1 Rabbit pAb (CAB10440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using LMAN1 Rabbit pAb (CAB10440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using LMAN1 Rabbit pAb (CAB10440) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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