Anti-LITAF Antibody (CAB5469)[KO Validated]

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SKU:
CAB5469
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-LITAF Antibody [KO Validated]
Antibody SKU:CAB5469
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LITAF (NP_004853.2).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:K-562, HeLa, HepG2, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LITAF (NP_004853.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVP GPYQ AATG PSSA PSAP PSYE ETVA VNSY YPTP PAPM PGPT TGLV TGPD GKGM NPPS YYTQ PAPI PNNN PITV QTVY VQHP ITFL DRPI QMCC PSCN KMIV SQLS YNAG ALTW LSCG SLCL LGCI AGCC FIPF CVDA LQDV DHYC PNCR ALLG TYKR L
Gene ID:9516
Uniprot:Q99732
Cellular Location:Cytoplasmic side, Lysosome membrane, Peripheral membrane protein
Calculated MW:15kDa/17kDa/23kDa
Observed MW:20kDa
Synonyms:LITAF, PIG7, SIMPLE, TP53I7
Background:Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:LITAF: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF- alpha) gene expression. Interacts with NEDD4. Interacts with WWOX. Isoform 2 may interact with STAT6. By bacterial lipopolysaccharides (LPS) or p53/TP53. In monocytes by the Bacillus Calmette-Guerin (BCG). Ubiquitously and abundantly expressed. Expressed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 16p13.13

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; plasma membrane

Molecular Function:signal transducer activity; protein binding; WW domain binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; negative regulation of NF-kappaB import into nucleus; signal transduction; regulation of cytokine production; aging

Disease: Charcot-marie-tooth Disease, Demyelinating, Type 1c

NCBI Summary:Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:Q99732
NCBI GenInfo Identifier:83304387
NCBI Gene ID:9516
NCBI Accession:Q99732.2
UniProt Secondary Accession:Q99732,Q05DW0, Q9C0L6, D3DUG1, G5E9K0,
UniProt Related Accession:Q99732
Molecular Weight:161
NCBI Full Name:Lipopolysaccharide-induced tumor necrosis factor-alpha factor
NCBI Synonym Full Names:lipopolysaccharide-induced TNF factor
NCBI Official Symbol:LITAF  
NCBI Official Synonym Symbols:PIG7; SIMPLE; TP53I7  
NCBI Protein Information:lipopolysaccharide-induced tumor necrosis factor-alpha factor; p53-induced gene 7 protein; LPS-induced TNF-alpha factor; tumor protein p53 inducible protein 7; lipopolysaccharide-induced TNF-alpha factor; small integral membrane protein of lysosome/late endosome
UniProt Protein Name:Lipopolysaccharide-induced tumor necrosis factor-alpha factor
UniProt Synonym Protein Names:Small integral membrane protein of lysosome/late endosome; p53-induced gene 7 protein
Protein Family:Lipopolysaccharide-induced tumor necrosis factor-alpha factor
UniProt Gene Name:LITAF  
UniProt Entry Name:LITAF_HUMAN
Anti-LITAF Antibody [KO Validated] (CAB5469)Western blot analysis of extracts of various cell lines, using LITAF antibody (CAB5469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-LITAF Antibody [KO Validated] (CAB5469)Western blot analysis of extracts from normal (control) and LITAF knockout (KO) HeLa cells, using LITAF antibody (CAB5469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-LITAF Antibody [KO Validated] (CAB5469)Immunohistochemistry of paraffin-embedded human prostate using LITAF Antibody (CAB5469) at dilution of 1:100 (40x lens).

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