Anti-LIPF Antibody (CAB7016)
- SKU:
- CAB7016
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-LIPF Antibody |
| Antibody SKU: | CAB7016 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 219-398 of human LIPF (NP_004181.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Mouse small intestine, Mouse lung, Mouse heart, Mouse liver, Mouse stomach, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 219-398 of human LIPF (NP_004181.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QSLF KFIF GDKI FYPH NFFD QFLA TEVC SREM LNLL CSNA LFII CGFD SKNF NTSR LDVY LSHN PAGT SVQN MFHW TQAV KSGK FQAY DWGS PVQN RMHY DQSQ PPYY NVTA MNVP IAVW NGGK DLLA DPQD VGLL LPKL PNLI YHKE IPFY NHLD FIWA MDAP QEVY NDIV SMIS EDKK |
| Gene ID: | 8513 |
| Uniprot: | P07098 |
| Cellular Location: | Secreted |
| Calculated MW: | 41kDa/42kDa/45kDa/46kDa |
| Observed MW: | 45kDa |
| Synonyms: | LIPF, GL, HGL, HLAL, lipase F |
| Background: | This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | LIPF: gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| UniProt Protein Details: | Protein type:EC 3.1.1.3; Secreted; Hydrolase; Secreted, signal peptide; Lipid Metabolism - glycerolipid Chromosomal Location of Human Ortholog: 10q23.31 Cellular Component: mitochondrion; extracellular region Molecular Function:triacylglycerol lipase activity; malate dehydrogenase activity; lipid binding Biological Process: malate metabolic process; triacylglycerol metabolic process; lipid catabolic process |
| NCBI Summary: | This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010] |
| UniProt Code: | P07098 |
| NCBI GenInfo Identifier: | 126306 |
| NCBI Gene ID: | 8513 |
| NCBI Accession: | P07098.1 |
| UniProt Secondary Accession: | P07098,Q2M1P6, Q5VXI7, Q5VXI8, Q658L8, B7Z723, F5H1P4 |
| UniProt Related Accession: | P07098 |
| Molecular Weight: | 398 |
| NCBI Full Name: | Gastric triacylglycerol lipase |
| NCBI Synonym Full Names: | lipase, gastric |
| NCBI Official Symbol: | LIPF |
| NCBI Official Synonym Symbols: | GL; HGL; HLAL |
| NCBI Protein Information: | gastric triacylglycerol lipase; gastric lipase |
| UniProt Protein Name: | Gastric triacylglycerol lipase |
| Protein Family: | Carboxylesterase |
| UniProt Gene Name: | LIPF |
| UniProt Entry Name: | LIPG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LIPF antibody (CAB7016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
Related Products
Human Gastric triacylglycerol lipase (LIPF) ELISA Kit
Mouse Gastric triacylglycerol lipase (Lipf) ELISA Kit
Rat Gastric triacylglycerol lipase (Lipf) ELISA Kit
