Anti-LIMS2 Antibody (CAB13225)
- SKU:
- CAB13225
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-LIMS2 Antibody |
| Antibody SKU: | CAB13225 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human LIMS2 (NP_001154875.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-549, HeLa, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-341 of human LIMS2 (NP_001154875.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTGS NMSD ALAN AVCQ RCQA RFSP AERI VNSN GELY HEHC FVCA QCFR PFPE GLFY EFEG RKYC EHDF QMLF APCC GSCG EFII GRVI KAMN NNWH PGCF RCEL CDVE LADL GFVK NAGR HLCR PCHN REKA KGLG KYIC QRCH LVID EQPL MFRS DAYH PDHF NCTH CGKE LTAE AREL KGEL YCLP CHDK MGVP ICGA CRRP IEGR VVNA LGKQ WHVE HFVC AKCE KPFL GHRH YEKK GLAY CETH YNQL FGDV CYNC SHVI EGDV VSAL NKAW CVSC FSCS TCNS KLTL KNKF VEFD MKPV CKRC YEKF PLEL KKRL KKLS ELTS RKAQ PKAT DLNS A |
| Gene ID: | 55679 |
| Uniprot: | Q7Z4I7 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic side, Nucleus, Peripheral membrane protein, focal adhesion |
| Calculated MW: | 21kDa/38kDa/41kDa |
| Observed MW: | 39kDa |
| Synonyms: | LIMS2, LGMD2W, PINCH-2, PINCH2 |
| Background: | This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | LIMS2: Competes with LIMS1 for binding to ILK. Plays a role in modulating cell spreading and migration. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 2q14.3 Cellular Component: cytosol; focal adhesion |
| NCBI Summary: | This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q7Z4I7 |
| NCBI GenInfo Identifier: | 74750091 |
| NCBI Gene ID: | 55679 |
| NCBI Accession: | Q7Z4I7.1 |
| UniProt Secondary Accession: | Q7Z4I7,Q7Z4I2, Q7Z4I6, Q7Z4I8, Q8NFE7, Q9HA13, A6NLH0 B4DMV1, F5H6E6, |
| UniProt Related Accession: | Q7Z4I7 |
| Molecular Weight: | 41,226 Da |
| NCBI Full Name: | LIM and senescent cell antigen-like-containing domain protein 2 |
| NCBI Synonym Full Names: | LIM zinc finger domain containing 2 |
| NCBI Official Symbol: | LIMS2 |
| NCBI Official Synonym Symbols: | LGMD2W; PINCH2; PINCH-2 |
| NCBI Protein Information: | LIM and senescent cell antigen-like-containing domain protein 2 |
| UniProt Protein Name: | LIM and senescent cell antigen-like-containing domain protein 2 |
| UniProt Synonym Protein Names: | LIM-like protein 2; Particularly interesting new Cys-His protein 2; PINCH-2 |
| Protein Family: | LIM and senescent cell antigen-like-containing domain protein |
| UniProt Gene Name: | LIMS2 |
| UniProt Entry Name: | LIMS2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LIMS2 antibody (CAB13225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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