Anti-LDLRAP1 Antibody (CAB7093)
- SKU:
- CAB7093
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-LDLRAP1 Antibody |
| Antibody SKU: | CAB7093 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 59-308 of human LDLRAP1 (NP_056442.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, 293T, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 59-308 of human LDLRAP1 (NP_056442.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EQPK GEEL SAAA IKRI VATA KASG KKLQ KVTL KVSP RGII LTDN LTNQ LIEN VSIY RISY CTAD KMHD KVFA YIAQ SQHN QSLE CHAF LCTK RKMA QAVT LTVA QAFK VAFE FWQV SKEE KEKR DKAS QEGG DVLG ARQD CTPS LKSL VATG NLLD LEET AKAP LSTV SANT TNMD EVPR PQAL SGSS VVWE LDDG LDEA FSRL AQSR TNPQ VLDT GLTA QDMH YAQC LSPV DWDK PDSS GTEQ DDLF SF |
| Gene ID: | 26119 |
| Uniprot: | Q5SW96 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 33kDa |
| Observed MW: | 36KDa |
| Synonyms: | LDLRAP1, ARH, ARH1, ARH2, FHCB1, FHCB2 |
| Background: | The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. |
| UniProt Protein Function: | LDLRAP1: Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. Defects in LDLRAP1 are the cause of autosomal recessive hypercholesterolemia (ARH). ARH is a disorder caused by defective internalization of LDL receptors (LDLR) in the liver. ARH has the clinical features of familial hypercholesterolemia (FH) homozygotes, including severely elevated plasma LDL cholesterol, tuberous and tendon xanthomata, and premature atherosclerosis. LDL receptor (LDLR) activity measured in skin fibroblasts is normal, as the LDL binding ability. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1p36.11 Cellular Component: recycling endosome; internal side of plasma membrane; AP-1 adaptor complex; axon; early endosome; neurofilament; basal plasma membrane; AP-2 adaptor complex; cytosol Molecular Function:phosphatidylinositol-4,5-bisphosphate binding; protein binding; clathrin binding; receptor signaling complex scaffold activity; low-density lipoprotein receptor binding; phosphotyrosine binding; beta-amyloid binding Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; cholesterol homeostasis; positive regulation of signal transduction; transport; amyloid precursor protein metabolic process; regulation of protein binding; receptor internalization; positive regulation of receptor-mediated endocytosis Disease: Hypercholesterolemia, Autosomal Recessive |
| NCBI Summary: | The protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q5SW96 |
| NCBI GenInfo Identifier: | 116241254 |
| NCBI Gene ID: | 26119 |
| NCBI Accession: | Q5SW96.3 |
| UniProt Related Accession: | Q5SW96 |
| Molecular Weight: | |
| NCBI Full Name: | Low density lipoprotein receptor adapter protein 1 |
| NCBI Synonym Full Names: | low density lipoprotein receptor adaptor protein 1 |
| NCBI Official Symbol: | LDLRAP1 |
| NCBI Official Synonym Symbols: | ARH; ARH1; ARH2; FHCB1; FHCB2; FHCL4 |
| NCBI Protein Information: | low density lipoprotein receptor adapter protein 1 |
| UniProt Protein Name: | Low density lipoprotein receptor adapter protein 1 |
| UniProt Synonym Protein Names: | Autosomal recessive hypercholesterolemia protein |
| UniProt Gene Name: | LDLRAP1 |
| UniProt Entry Name: | ARH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LDLRAP1 antibody (CAB7093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry of paraffin-embedded rat brain using LDLRAP1 antibody (CAB7093) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using LDLRAP1 antibody (CAB7093) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A-549 cells using LDLRAP1 antibody (CAB7093). |
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