Anti-LDHD Antibody (CAB15965)

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SKU:
CAB15965
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-LDHD Antibody
Antibody SKU:CAB15965
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 158-507 of human LDHD (NP_705690.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, U-87MG, HT-29, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 158-507 of human LDHD (NP_705690.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PGAD ASLC GMAA TGAS GTNA VRYG TMRD NVLN LEVV LPDG RLLH TAGR GRHF RFGF WPEI PHHT AWYS PCVS LGRR KSAA GYNL TGLF VGSE GTLG LITA TTLR LHPA PEAT VAAT CAFP SVQA AVDS TVHI LQAA VPVA RIEF LDEV MMDA CNRY SKLN CLVA PTLF LEFH GSQQ ALEE QLQR TEEI VQQN GASD FSWA KEAE ERSR LWTA RHNA WYAA LATR PGCK GYST DVCV PISR LPEI VVQT KEDL NASG LTGS IVGH VGDG NFHC ILLV NPDD AEEL GRVK AFAE QLGR RALA LHGT CTGE HGIG MGKR QLLQ EEVG AVGV ETMR QLKA VLDP QGLM NPGK VL
Gene ID:197257
Uniprot:Q86WU2
Cellular Location:Mitochondrion
Calculated MW:52kDa/54kDa
Observed MW:60kDa
Synonyms:LDHD, DLD
Background:The protein encoded by this gene belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
UniProt Protein Function:LDH-D: belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Protein Details:

Protein type:Mitochondrial; Carbohydrate Metabolism - pyruvate; EC 1.1.2.4; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function:D-lactate dehydrogenase (cytochrome) activity; FAD binding; protein binding

NCBI Summary:The protein encoded by this gene belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q86WU2
NCBI GenInfo Identifier:74727712
NCBI Gene ID:197257
NCBI Accession:Q86WU2.1
UniProt Secondary Accession:Q86WU2,Q8IZK5,
UniProt Related Accession:Q86WU2
Molecular Weight:52,144 Da
NCBI Full Name:Probable D-lactate dehydrogenase, mitochondrial
NCBI Synonym Full Names:lactate dehydrogenase D
NCBI Official Symbol:LDHD  
NCBI Official Synonym Symbols:DLD  
NCBI Protein Information:probable D-lactate dehydrogenase, mitochondrial
UniProt Protein Name:Probable D-lactate dehydrogenase, mitochondrial
Protein Family:Probable D-lactate dehydrogenase
UniProt Gene Name:LDHD  
UniProt Entry Name:LDHD_HUMAN
Anti-LDHD Antibody (CAB15965)Western blot analysis of extracts of various cell lines, using LDHD antibody (CAB15965) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

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