Anti-LCN2 Antibody (CAB11130)
- SKU:
- CAB11130
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-LCN2 Antibody |
| Antibody SKU: | CAB11130 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human LCN2 |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant protein of human LCN2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3934 |
| Uniprot: | P80188 |
| Cellular Location: | Secreted |
| Calculated MW: | 22kDa |
| Observed MW: | 23kDa |
| Synonyms: | LCN2, 24p3, MSFI, NGAL, p25 |
| Background: | This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. |
| UniProt Protein Function: | LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 9q34 Cellular Component: extracellular space; extracellular region; cytosol Molecular Function:protein homodimerization activity; protease binding; transporter activity; iron ion binding Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport |
| UniProt Code: | P80188 |
| NCBI GenInfo Identifier: | 1171700 |
| NCBI Gene ID: | 3934 |
| NCBI Accession: | P80188.2 |
| UniProt Secondary Accession: | P80188,P30150, Q5SYV9, Q5SYW0, Q6FGL5, Q92683, A6NII8 B4DWV4, B7ZAA2, |
| UniProt Related Accession: | P80188 |
| Molecular Weight: | |
| NCBI Full Name: | Neutrophil gelatinase-associated lipocalin |
| NCBI Synonym Full Names: | lipocalin 2 |
| NCBI Official Symbol: | LCN2 |
| NCBI Official Synonym Symbols: | 24p3; MSFI; NGAL |
| NCBI Protein Information: | neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 |
| UniProt Protein Name: | Neutrophil gelatinase-associated lipocalin |
| UniProt Synonym Protein Names: | 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25 |
| Protein Family: | Neutrophil gelatinase-associated lipocalin |
| UniProt Gene Name: | LCN2 |
| UniProt Entry Name: | NGAL_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using LCN2 antibody (CAB11130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of A-549 cells using LCN2 antibody (CAB11130). Blue: DAPI for nuclear staining. |
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