Anti-LC3B Antibody (CAB7198)[KO Validated]

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SKU:
CAB7198
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy
Frequently bought together:

Description

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Antibody Name:Anti-LC3B Antibody [KO Validated]
Antibody SKU:CAB7198
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human LC3B
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:C6, HeLa, NIH/3T3, Pig liver
Immunogen:A synthetic peptide of human LC3B
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:81631
Uniprot:Q9GZQ8
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:14kDa
Observed MW:14kDa, 16kDa
Synonyms:LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B
Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
NCBI Summary:The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZQ8
NCBI GenInfo Identifier:17433141
NCBI Gene ID:81631
NCBI Accession:Q9GZQ8.3
UniProt Related Accession:Q9GZQ8
Molecular Weight:
NCBI Full Name:Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:MAP1LC3B  
NCBI Official Synonym Symbols:LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3  
NCBI Protein Information:microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:MAP1LC3B  
UniProt Entry Name:MLP3B_HUMAN
Anti-LC3B Antibody [KO Validated] (CAB7198)Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB7198) at 1:1000 dilution. C6 cells were treated by Chloroquine (50 uM) for 20 hours. Hela cells were treated by Chloroquine (50 uM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-LC3B Antibody [KO Validated] (CAB7198)Western blot analysis of extracts of pig liver, using LC3B antibody (CAB7198). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-LC3B Antibody [KO Validated] (CAB7198)Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (CAB7198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-LC3B Antibody [KO Validated] (CAB7198)Immunohistochemistry of paraffin-embedded rat brain using LC3B antibody (CAB7198) at dilution of 1:100 (40x lens).
Anti-LC3B Antibody [KO Validated] (CAB7198)Immunohistochemistry of paraffin-embedded human breast cancer using LC3B antibody (CAB7198) at dilution of 1:100 (40x lens).

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