Anti-L3MBTL3 Antibody (CAB7289)[KO Validated]

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SKU:
CAB7289
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-L3MBTL3 Antibody [KO Validated]
Antibody SKU:CAB7289
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTES ASST SGQE FDVF SVMD WKDG VGTL PGSD LKFR VNEF GALE VITD ENEM ENVK KATA TTTW MVPT AQEV FSEK TGMP FRLK DPVK VEGL QFCE NCCQ YGNV DECL SGGN YCSQ NCAR HIKD KDQK EERD VEED NEEE DPKC SRKK KPKL SLKA DTKE DGEE RDDE MENK QDVR ILRG SQRA RRKR RGDS AVLK QGLP PKGK KAWC WASY LEEE KAVA VPAK LFKE HQ
Gene ID:84456
Uniprot:Q96JM7
Cellular Location:Nucleus
Calculated MW:85kDa/88kDa
Observed MW:100KDa
Synonyms:L3MBTL3, MBT-1, MBT1
Background:This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma. [provided by RefSeq, Oct 2016]
UniProt Protein Function:L3MBTL3: a Polycomb group (PcG) protein that regulates the maturation of myeloid progenitor cells. May influence myelopoiesis by transiently enhancing p57(KIP2) expression levels. PcG proteins participate in DNA-binding complexes with gene-repressing activity, many of which have been highlighted for their involvement in hematopoiesis. A member of the malignant brain tumor (MBT) family of chromatin-interacting transcriptional repressors. Contains a SAM domain, a common protein modules found in eukaryotic proteomes. Many SAM domains have been shown to form helical polymer structures. May create large protein complexes in the cell. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription regulation

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: nucleus

Biological Process: macrophage differentiation; erythrocyte maturation; granulocyte differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification

UniProt Code:Q96JM7
NCBI GenInfo Identifier:62900619
NCBI Gene ID:84456
NCBI Accession:Q96JM7.2
UniProt Secondary Accession:Q96JM7,Q4VXE1, Q5VUM9, Q6P9B5,
UniProt Related Accession:Q96JM7
Molecular Weight:85,757 Da
NCBI Full Name:Lethal(3)malignant brain tumor-like protein 3
NCBI Synonym Full Names:l(3)mbt-like 3 (Drosophila)
NCBI Official Symbol:L3MBTL3  
NCBI Official Synonym Symbols:MBT1; MBT-1  
NCBI Protein Information:lethal(3)malignant brain tumor-like protein 3; lethal(3)malignant brain tumor-like protein 3; H-l(3)mbt-like protein 3; l(3)mbt-like protein 3
UniProt Protein Name:Lethal(3)malignant brain tumor-like protein 3
UniProt Synonym Protein Names:MBT-1
Protein Family:Lethal(3)malignant brain tumor-like protein
UniProt Gene Name:L3MBTL3  
UniProt Entry Name:LMBL3_HUMAN
Anti-L3MBTL3 Antibody [KO Validated] (CAB7289)Western blot analysis of extracts of 293T cells, using L3MBTL3 antibody (CAB7289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-L3MBTL3 Antibody [KO Validated] (CAB7289)Immunofluorescence analysis of MCF-7 cells using L3MBTL3 antibody (CAB7289). Blue: DAPI for nuclear staining.
Anti-L3MBTL3 Antibody [KO Validated] (CAB7289)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug L3MBTL3 antibody (CAB7289). Western blot was performed from the immunoprecipitate using L3MBTL3 antibody (CAB7289) at a dilition of 1:1000.

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