Anti-KRT5 Antibody (CAB2662)
- SKU:
- CAB2662
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-KRT5 Antibody |
| Antibody SKU: | CAB2662 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human KRT5 |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-431, Mouse kidney, Mouse thymus, Mouse skeletal muscle |
| Immunogen: | Recombinant protein of human KRT5 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3852 |
| Uniprot: | P13647 |
| Cellular Location: | |
| Calculated MW: | 62kDa |
| Observed MW: | 70kDa |
| Synonyms: | KRT5, CK5, DDD, DDD1, EBS2, K5, KRT5A, keratin 5 |
| Background: | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
| UniProt Protein Function: | K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: mitochondrion; membrane; cytoplasm; keratin filament; plasma membrane; intermediate filament; cytosol; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: epidermis development; hemidesmosome assembly Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type |
| NCBI Summary: | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13647 |
| NCBI GenInfo Identifier: | 143811411 |
| NCBI Gene ID: | 3852 |
| NCBI Accession: | P13647.3 |
| UniProt Secondary Accession: | P13647,Q6PI71, Q6UBJ0, Q8TA91, |
| UniProt Related Accession: | P13647 |
| Molecular Weight: | 590 |
| NCBI Full Name: | Keratin, type II cytoskeletal 5 |
| NCBI Synonym Full Names: | keratin 5 |
| NCBI Official Symbol: | KRT5 |
| NCBI Official Synonym Symbols: | K5; CK5; DDD; DDD1; EBS2; KRT5A |
| NCBI Protein Information: | keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) |
| UniProt Protein Name: | Keratin, type II cytoskeletal 5 |
| UniProt Synonym Protein Names: | 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5 |
| Protein Family: | Keratin |
| UniProt Gene Name: | KRT5 |
| UniProt Entry Name: | K2C5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KRT5 antibody (CAB2662) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Human esophageal cancer using KRT5 Rabbit pAb (CAB2662) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using KRT5 Rabbit pAb (CAB2662) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug KRT5 antibody (CAB2662). Western blot was performed from the immunoprecipitate using KRT5 antibody (CAB2662) at a dilition of 1:5000. |
Related Products
