Anti-KLHL8 Antibody (CAB14917)
- SKU:
- CAB14917
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-KLHL8 Antibody |
| Antibody SKU: | CAB14917 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse brain, Mouse kidney, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 271-620 of human KLHL8 (NP_065854.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AKEQ IVKQ NLKC RDLL DEAR NYHL HLSS RAVP DFEY SIRT TPRK HTAG VLFC VGGR GGSG DPFR SIEC YSIN KNSW FFGP EMNS RRRH VGVI SVEG KVYA VGGH DGNE HLGS MEMF DPLT NKWM MKAS MNTK RRGI ALAS LGGP IYAI GGLD DNTC FNDV ERYD IESD QWST VAPM NTPR GGVG SVAL VNHV YAVG GNDG MASL SSVE RYDP HLDK WIEV KEMG QRRA GNGV SKLH GCLY VVGG FDDN SPLS SVER YDPR SNKW DYVA ALTT PRGG VGIA TVMG KIFA VGGH NGNA YLNT VEAF DPVL NRWE LVGS VSHC RAGA GVAV CSCL TSQI RDVG HGSN NVVD CM |
| Gene ID: | 57563 |
| Uniprot: | Q9P2G9 |
| Cellular Location: | |
| Calculated MW: | 60kDa/68kDa |
| Observed MW: | 69kDa |
| Synonyms: | KLHL8 |
| Background: |
| UniProt Protein Function: | KLHL8: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN. Component of the BCR(KLHL8) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL8 and RBX1. Interacts with RAPSN |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 4q22.1 Cellular Component: nucleoplasm Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination |
| UniProt Code: | Q9P2G9 |
| NCBI GenInfo Identifier: | 635172825 |
| NCBI Gene ID: | 57563 |
| NCBI Accession: | NP_001278932.1 |
| UniProt Related Accession: | Q9P2G9 |
| Molecular Weight: | 60kDa; 68kDa |
| NCBI Full Name: | kelch-like protein 8 isoform 1 |
| NCBI Synonym Full Names: | kelch like family member 8 |
| NCBI Official Symbol: | KLHL8 |
| NCBI Protein Information: | kelch-like protein 8 |
| UniProt Protein Name: | Kelch-like protein 8 |
| Protein Family: | Kelch-like protein |
| UniProt Gene Name: | KLHL8 |
| UniProt Entry Name: | KLHL8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KLHL8 antibody (CAB14917) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using KLHL8 antibody (CAB14917) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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