Anti-KIF5A Antibody (CAB3303)
- SKU:
- CAB3303
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-KIF5A Antibody |
| Antibody SKU: | CAB3303 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 933-1032 of human KIF5A (NP_004975.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TNPY GTRS PECI SYTN SLFQ NYQN LYLQ ATPS STSD MYFA NSCT SSGA TSSG GPLA SYQK ANMD NGNA TDIN DNRS DLPC GYEA EDQA KLFP LHQE TAAS |
| Gene ID: | 3798 |
| Uniprot: | Q12840 |
| Cellular Location: | Cytoplasm, cytoskeleton, perinuclear region |
| Calculated MW: | 117kDa |
| Observed MW: | 129kDa |
| Synonyms: | KIF5A, D12S1889, MY050, NEIMY, NKHC, SPG10 |
| Background: | This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. |
| UniProt Protein Function: | KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily. |
| UniProt Protein Details: | Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: kinesin complex; microtubule; membrane; perinuclear region of cytoplasm; cytosol Molecular Function:protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding Biological Process: synaptic transmission; axon guidance; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement Disease: Spastic Paraplegia 10, Autosomal Dominant |
| NCBI Summary: | This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12840 |
| NCBI GenInfo Identifier: | 143811412 |
| NCBI Gene ID: | 3798 |
| NCBI Accession: | Q12840.2 |
| UniProt Secondary Accession: | Q12840,Q4LE26, A6H8M5, |
| UniProt Related Accession: | Q12840 |
| Molecular Weight: | 117,378 Da |
| NCBI Full Name: | Kinesin heavy chain isoform 5A |
| NCBI Synonym Full Names: | kinesin family member 5A |
| NCBI Official Symbol: | KIF5A |
| NCBI Official Synonym Symbols: | NKHC; MY050; SPG10; D12S1889 |
| NCBI Protein Information: | kinesin heavy chain isoform 5A; KIF5A variant protein; neuronal kinesin heavy chain; kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific |
| UniProt Protein Name: | Kinesin heavy chain isoform 5A |
| UniProt Synonym Protein Names: | Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; NKHC |
| Protein Family: | Kinesin |
| UniProt Gene Name: | KIF5A |
| UniProt Entry Name: | KIF5A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIF5A antibody (CAB3303) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using KIF5A antibody (CAB3303) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of PC-12 cells using KIF5A Rabbit pAb (CAB3303) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of SH-SY5Y cells using KIF5A Rabbit pAb (CAB3303) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
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