Anti-KIF3A Antibody (CAB6639)
- SKU:
- CAB6639
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-KIF3A Antibody |
| Antibody SKU: | CAB6639 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF-7, SW480, 293T, SKOV3, HeLa, Mouse brain, Mouse ovary, Rat liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RKAL ETKL DMEE EERN KARA ELEK REKD LLKA QQEH QSLL EKLS ALEK KVIV GGVD LLAK AEEQ EKLL EESN MELE ERRK RAEQ LRRE LEEK EQER LDIE EKYT SLQE EAQG KTKK LKKV WTML MAAK SEMA DLQQ EHQR EIEG LLEN IRQL SREL RLQM LIID NFIP RDYQ EMIE NYVH WNED IGEW QLKC VAYT GNNM RKQT PVPD KKEK DPFE VDLS HVYL AYTE ESLR QSLM KLER PRTS KGKA RPKT GRRK RSAK PETV IDSL LQ |
| Gene ID: | 11127 |
| Uniprot: | Q9Y496 |
| Cellular Location: | Cell projection, Cytoplasm, cilium, cytoskeleton |
| Calculated MW: | 80kDa |
| Observed MW: | 80kDa |
| Synonyms: | KIF3A, FLA10, KLP-20 |
| Background: |
| UniProt Protein Function: | KIF3A: Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Heterodimer of KIF3A and KIF3B. Interacts with PIFO/C1orf88. Interacts with CLN3. Belongs to the kinesin-like protein family. Kinesin II subfamily. |
| UniProt Protein Details: | Protein type:Microtubule-binding; Motor Chromosomal Location of Human Ortholog: 5q31 Cellular Component: centrosome; cytosol; kinesin II complex; microtubule cytoskeleton; spindle microtubule Molecular Function:plus-end-directed microtubule motor activity; protein binding; spectrin binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cilium biogenesis; microtubule-based movement; neurogenesis; organelle organization and biogenesis; retrograde vesicle-mediated transport, Golgi to ER |
| UniProt Code: | Q9Y496 |
| NCBI GenInfo Identifier: | 296439481 |
| NCBI Gene ID: | 11127 |
| NCBI Accession: | Q9Y496.4 |
| UniProt Secondary Accession: | Q9Y496,Q59EN1, Q86XE9, Q9Y6V4, A8MSW9, |
| UniProt Related Accession: | Q9Y496 |
| Molecular Weight: | 80,041 Da |
| NCBI Full Name: | Kinesin-like protein KIF3A |
| NCBI Synonym Full Names: | kinesin family member 3A |
| NCBI Official Symbol: | KIF3A |
| NCBI Official Synonym Symbols: | FLA10; KLP-20 |
| NCBI Protein Information: | kinesin-like protein KIF3A |
| UniProt Protein Name: | Kinesin-like protein KIF3A |
| UniProt Synonym Protein Names: | Microtubule plus end-directed kinesin motor 3A |
| Protein Family: | Kinesin-like protein |
| UniProt Gene Name: | KIF3A |
| UniProt Entry Name: | KIF3A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIF3A antibody (CAB6639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF7 cells using KIF3A antibody (CAB6639). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug KIF3A antibody (CAB6639). Western blot was performed from the immunoprecipitate using KIF3A antibody (CAB6639) at a dilition of 1:1000. |
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