Anti-KIF2C Antibody (CAB5449)
- SKU:
- CAB5449
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-KIF2C Antibody |
| Antibody SKU: | CAB5449 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human KIF2C (NP_006836.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, Lovo, K-562, HeLa, OVCAR3, U-937, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human KIF2C (NP_006836.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAMD SSLQ ARLF PGLA IKIQ RSNG LIHS ANVR TVNL EKSC VSVE WAEG GATK GKEI DFDD VAAI NPEL LQLL PLHP KDNL PLQE NVTI QKQK RRSV NSKI PAPK ESLR SRST RMST VSEL RITA QEND MEVE LPAA ANSR KQFS VPPA PTRP SCPA VAEI PLRM VSEE MEEQ VHSI RGSS SANP VNSV RR |
| Gene ID: | 11004 |
| Uniprot: | Q99661 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore |
| Calculated MW: | 75kDa/81kDa |
| Observed MW: | 95kDa |
| Synonyms: | KIF2C, CT139, KNSL6, MCAK |
| Background: | This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | KIF2C: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cancer Testis Antigen (CTA); Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: chromosome, pericentric region; cytoplasmic microtubule; cytosol; kinesin complex; kinetochore; membrane; microtubule cytoskeleton Molecular Function:ATPase activity; centromeric DNA binding; microtubule plus-end binding; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell proliferation; establishment and/or maintenance of microtubule cytoskeleton polarity; microtubule depolymerization; microtubule-based movement; mitosis; mitotic metaphase plate congression; regulation of chromosome segregation; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q99661 |
| NCBI GenInfo Identifier: | 20141607 |
| NCBI Gene ID: | 11004 |
| NCBI Accession: | Q99661.2 |
| UniProt Secondary Accession: | Q99661,Q5JR88, Q6ICU1, Q96C18, Q96HB8, Q9BWV8, B3ITR9 |
| UniProt Related Accession: | Q99661 |
| Molecular Weight: | 75,561 Da |
| NCBI Full Name: | Kinesin-like protein KIF2C |
| NCBI Synonym Full Names: | kinesin family member 2C |
| NCBI Official Symbol: | KIF2C |
| NCBI Official Synonym Symbols: | MCAK; CT139; KNSL6 |
| NCBI Protein Information: | kinesin-like protein KIF2C |
| UniProt Protein Name: | Kinesin-like protein KIF2C |
| UniProt Synonym Protein Names: | Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK |
| Protein Family: | Kinesin-like protein |
| UniProt Gene Name: | KIF2C |
| UniProt Entry Name: | KIF2C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIF2C antibody (CAB5449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded Rat testis using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human stomach using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse testis using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using KIF2C antibody (CAB5449). Blue: DAPI for nuclear staining. |
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