Anti-KIF20A Antibody (CAB15377)
- SKU:
- CAB15377
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-KIF20A Antibody |
| Antibody SKU: | CAB15377 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 591-890 of human KIF20A (NP_005724.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, A-549, Mouse spleen, Mouse testis, Mouse heart, Mouse lung, Mouse brain, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 591-890 of human KIF20A (NP_005724.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EICN EMVE QMQQ REQW CSEH LDTQ KELL EEMY EEKL NILK ESLT SFYQ EEIQ ERDE KIEE LEAL LQEA RQQS VAHQ QSGS ELAL RRSQ RLAA SAST QQLQ EVKA KLQQ CKAE LNST TEEL HKYQ KMLE PPPS AKPF TIDV DKKL EEGQ KNIR LLRT ELQK LGES LQSA ERAC CHST GAGK LRQA LTTC DDIL IKQD QTLA ELQN NMVL VKLD LRKK AACI AEQY HTVL KLQG QVSA KKRL GTNQ ENQQ PNQQ PPGK KPFL RNLL PRTP TCQS STDC SPYA RILR SRRS PLLK SGPF GKKY |
| Gene ID: | 10112 |
| Uniprot: | O95235 |
| Cellular Location: | Cytoplasm, Golgi apparatus, cytoskeleton, spindle |
| Calculated MW: | 98kDa/100kDa |
| Observed MW: | 105kDa |
| Synonyms: | KIF20A, MKLP2, RAB6KIFL |
| Background: |
| UniProt Protein Function: | KIF20A: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Belongs to the kinesin-like protein family. |
| UniProt Protein Details: | Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 5q31 Cellular Component: kinesin complex; midbody; nucleoplasm; spindle Molecular Function:ATPase activity; microtubule motor activity; protein binding; protein kinase binding; transporter activity Biological Process: cell separation during cytokinesis; cytokinesis; cytokinesis after mitosis; microtubule bundle formation; microtubule-based movement; vesicle-mediated transport |
| UniProt Code: | O95235 |
| NCBI GenInfo Identifier: | 6225915 |
| NCBI Gene ID: | 10112 |
| NCBI Accession: | O95235.1 |
| UniProt Secondary Accession: | O95235,B4DL79, D3DQB6, |
| UniProt Related Accession: | O95235 |
| Molecular Weight: | 98,100 Da |
| NCBI Full Name: | Kinesin-like protein KIF20A |
| NCBI Synonym Full Names: | kinesin family member 20A |
| NCBI Official Symbol: | KIF20A |
| NCBI Official Synonym Symbols: | MKLP2; RAB6KIFL |
| NCBI Protein Information: | kinesin-like protein KIF20A |
| UniProt Protein Name: | Kinesin-like protein KIF20A |
| UniProt Synonym Protein Names: | GG10_2; Mitotic kinesin-like protein 2; MKlp2; Rab6-interacting kinesin-like protein; Rabkinesin-6 |
| Protein Family: | Kinesin-like protein |
| UniProt Gene Name: | KIF20A |
| UniProt Entry Name: | KI20A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KIF20A antibody (CAB15377) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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