Anti-Ki67 Antibody (CAB11390)
- SKU:
- CAB11390
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-Ki67 Antibody |
| Antibody SKU: | CAB11390 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Ki67 (NP_001139438.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 22Rv1, Raji |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Ki67 (NP_001139438.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GDGK SIRT FKES PKQI LDPA ARVT GMKK WPRT PKEE AQSL EDLA GFKE LFQT PGPS EESM TDEK TTKI ACKS PPPE SVDT PTST KQWP KRSL RKAD VEEE F |
| Gene ID: | 4288 |
| Uniprot: | P46013 |
| Cellular Location: | Chromosome, Nucleus, nucleolus |
| Calculated MW: | 319kDa/358kDa |
| Observed MW: | 359kDa |
| Synonyms: | MKI67, KIA, MIB-, MIB-1, PPP1R105, marker of proliferation Ki-67, Ki67 |
| Background: | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. |
| UniProt Protein Function: | KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. |
| UniProt Protein Details: | Protein type:Nucleolus; Cell cycle regulation Chromosomal Location of Human Ortholog: 10q26.2 Cellular Component: chromosome, pericentric region; condensed chromosome; cytoplasm; membrane; nucleolus; nucleus Molecular Function:ATP binding; protein binding; protein C-terminus binding Biological Process: cell proliferation; DNA metabolic process; hyaluronan metabolic process; meiosis; organ regeneration; response to organic cyclic substance |
| NCBI Summary: | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P46013 |
| NCBI GenInfo Identifier: | 118572663 |
| NCBI Gene ID: | 4288 |
| NCBI Accession: | P46013.2 |
| UniProt Secondary Accession: | P46013,Q5VWH2, |
| UniProt Related Accession: | P46013 |
| Molecular Weight: | 319,444 Da |
| NCBI Full Name: | Antigen KI-67 |
| NCBI Synonym Full Names: | marker of proliferation Ki-67 |
| NCBI Official Symbol: | MKI67 |
| NCBI Official Synonym Symbols: | KIA; MIB-; MIB-1; PPP1R105 |
| NCBI Protein Information: | antigen KI-67 |
| UniProt Protein Name: | Antigen KI-67 |
| UniProt Gene Name: | MKI67 |
| UniProt Entry Name: | KI67_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Ki67 antibody (CAB11390) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using Ki67 antibody (CAB11390). |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-CHD1L Antibody (CAB17558)
