Anti-KDM3A Antibody (CAB11960)
- SKU:
- CAB11960
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-KDM3A Antibody |
| Antibody SKU: | CAB11960 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human KDM3A (NP_060903.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human KDM3A (NP_060903.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LNTK SEAL RTKP DVCK AGLL SKSS QIGT GDLK ILTE PKGS CTQP KTNT DQEN RLES VPQA LTGL PKEC LPTK ASSK AELE IANP PELQ KHLE HAPS PSDV S |
| Gene ID: | 55818 |
| Uniprot: | Q9Y4C1 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 147kDa |
| Observed MW: | 147kDa |
| Synonyms: | KDM3A, JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA |
| Background: | This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family. |
| UniProt Protein Details: | Protein type:EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus Molecular Function:androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| UniProt Code: | Q9Y4C1 |
| NCBI GenInfo Identifier: | 308153659 |
| NCBI Gene ID: | 55818 |
| NCBI Accession: | Q9Y4C1.4 |
| UniProt Related Accession: | Q9Y4C1 |
| Molecular Weight: | 147kDa |
| NCBI Full Name: | Lysine-specific demethylase 3A |
| NCBI Synonym Full Names: | lysine demethylase 3A |
| NCBI Official Symbol: | KDM3A |
| NCBI Official Synonym Symbols: | TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A |
| NCBI Protein Information: | lysine-specific demethylase 3A |
| UniProt Protein Name: | Lysine-specific demethylase 3A |
| UniProt Synonym Protein Names: | JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A |
| Protein Family: | Lysine-specific demethylase |
| UniProt Gene Name: | KDM3A |
| UniProt Entry Name: | KDM3A_HUMAN |
 | Western blot analysis of extracts of 293T cells, using KDM3A Antibody (CAB11960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse testis using KDM3A antibody (CAB11960) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using KDM3A antibody (CAB11960) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using KDM3A Polyclonal Antibody (CAB11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using KDM3A Polyclonal Antibody (CAB11960) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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