Anti-KDM1 Antibody (CAB15794)[KO Validated]

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SKU:
CAB15794
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-KDM1 Antibody [KO Validated]
Antibody SKU:CAB15794
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, HeLa, Jurkat, Mouse liver, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSED EYYS EEER NAKA EKEK KLPP PPPQ APPE EENE SEPE EPSG VEGA AFQS RLPH DRMT SQEA ACFP DIIS GPQQ TQKV FLFI RNRT LQLW LDNP KIQL TFEA TLQQ LEAP YNSD TVLV HRVH SYLE RHGL INFG IYKR IKPL PTKK TGKV IIIG SGVS GLAA ARQL QSFG MDVT LLEA RDRV GGRV ATFR KGNY VADL GAMV VTGL GGNP MAVV SKQV NMEL AKIK QKCP LYEA NGQA VPKE KDEM VEQ
Gene ID:23028
Uniprot:O60341
Cellular Location:Nucleus
Calculated MW:92kDa/95kDa
Observed MW:110kDa
Synonyms:KDM1A, AOF2, BHC110, CPRF, KDM1, LSD1, KDM1
Background:This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
UniProt Code:O60341
NCBI GenInfo Identifier:58761544
NCBI Gene ID:23028
NCBI Accession:NP_001009999.1
UniProt Secondary Accession:O60341,Q5TH94, Q5TH95, Q86VT7, Q8IXK4, Q8NDP6, Q8TAZ3 Q96AW4, A8MWP9,
UniProt Related Accession:O60341
Molecular Weight:852
NCBI Full Name:lysine-specific histone demethylase 1A isoform a
NCBI Synonym Full Names:lysine (K)-specific demethylase 1A
NCBI Official Symbol:KDM1A  
NCBI Official Synonym Symbols:AOF2; KDM1; LSD1; BHC110  
NCBI Protein Information:lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-con
UniProt Protein Name:Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names:BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
Protein Family:Lysine-specific histone demethylase
UniProt Gene Name:KDM1A  
UniProt Entry Name:KDM1A_HUMAN
Anti-KDM1 Antibody [KO Validated] (CAB15794)Western blot analysis of extracts of various cell lines, using KDM1 antibody (CAB15794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-KDM1 Antibody [KO Validated] (CAB15794)Western blot analysis of extracts from normal (control) and KDM1 Rabbit pAb knockout (KO) 293T cells, using KDM1 Rabbit pAb antibody (CAB15794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-KDM1 Antibody [KO Validated] (CAB15794)Immunohistochemistry of paraffin-embedded rat spleen using KDM1 antibody (CAB15794) at dilution of 1:100 (40x lens).
Anti-KDM1 Antibody [KO Validated] (CAB15794)Immunohistochemistry of paraffin-embedded human esophageal using KDM1 antibody (CAB15794) at dilution of 1:100 (40x lens).
Anti-KDM1 Antibody [KO Validated] (CAB15794)Immunohistochemistry of paraffin-embedded mouse brain using KDM1 antibody (CAB15794) at dilution of 1:100 (40x lens).

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