Anti-KAT7 Antibody (CAB5823)
- SKU:
- CAB5823
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-KAT7 Antibody |
| Antibody SKU: | CAB5823 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-193 of human KAT7 (NP_008998.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | BT-474, HepG2, 293T, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-193 of human KAT7 (NP_008998.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSSP VRNL QSFG TEEP AYST RRVT RSQQ QPTP VTPK KYPL RQTR SSGS ETEQ VVDF SDRE TKNT ADHD ESPP RTPT GNAP SSES DIDI SSPN VSHD ESIA KDMS LKDS GSDL SHRP KRRR FHES YNFN MKCP TPGC NSL |
| Gene ID: | 11143 |
| Uniprot: | O95251 |
| Cellular Location: | Nucleus, nucleoplasm |
| Calculated MW: | 51kDa/55kDa/58kDa/66kDa/70kDa |
| Observed MW: | 63kDa |
| Synonyms: | KAT7, HBO1, HBOA, MYST2, ZC2HC7 |
| Background: | The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed. |
| UniProt Protein Function: | MYST2: a histone acetyltransferase that participates in DNA replication and the loading of the minichromosome maintenance (Mcm) complex onto chromatin. Is required for origin recognition complex (Orc) formation and replication licensing, and is responsible for much of the acetylation of histone H4. Interacts with MCM2 and ORC1L. Phosphorylated by Cdc2 during mitosis, creating a site that recruits Plk1, which in turn phosphorylates MYST2. Its phosphorylation by Plk1 during mitosis may be required for the formation of the prereplicative complex. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone. Specifically represses AR-mediated transcription. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Acetyltransferase Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: cytoplasm; histone acetyltransferase complex; nucleolus; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: DNA replication |
| UniProt Code: | O95251 |
| NCBI GenInfo Identifier: | 68565854 |
| NCBI Gene ID: | 11143 |
| NCBI Accession: | O95251.1 |
| UniProt Secondary Accession: | O95251,B3KN74, B4DF85, B4DFB4, B4DFE0, B4DGY4, E7ER15 G5E9K7, |
| UniProt Related Accession: | O95251 |
| Molecular Weight: | 55,135 Da |
| NCBI Full Name: | Histone acetyltransferase KAT7 |
| NCBI Synonym Full Names: | lysine acetyltransferase 7 |
| NCBI Official Symbol: | KAT7 |
| NCBI Official Synonym Symbols: | HBO1; HBOA; MYST2; ZC2HC7 |
| NCBI Protein Information: | histone acetyltransferase KAT7 |
| UniProt Protein Name: | Histone acetyltransferase KAT7 |
| UniProt Synonym Protein Names: | Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST-2 |
| Protein Family: | Histone acetyltransferase |
| UniProt Gene Name: | KAT7 |
| UniProt Entry Name: | KAT7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using KAT7 antibody (CAB5823) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using KAT7 Rabbit pAb (CAB5823) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using KAT7 Rabbit pAb (CAB5823) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using KAT7 Rabbit pAb (CAB5823) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using KAT7 Rabbit pAb (CAB5823) at dilution of 1:100 (40x lens). |
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