Anti-IVL Antibody (CAB8026)
- SKU:
- CAB8026
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | IVL Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8026 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-585 of human IVL (NP_005538.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A375, Jurkat, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-585 of human IVL (NP_005538.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QERQ VEHL EQQV GQLK HLEE QEGQ LKHL EQQQ GQLE VPEQ QVGQ PKNL EQEE KQLE LPEQ QEGQ VKHL EKQE AQLE LPEQ QVGQ PKHL EQQE KHLE HPEQ QDGQ LKHL EQQE GQLK DLEQ QKGQ LEQP VFAP APGQ VQDI QPAL PTKG EVLL PVEH QQQK QEVQ WPPK HK |
| Gene ID: | 3713 |
| Uniprot: | P07476 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 68kDa |
| Observed MW: | 80kDa |
| Synonyms: | IVL |
| Background: | Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. |
| UniProt Protein Function: | IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytoplasm; cornified envelope Molecular Function:protein binding, bridging; structural molecule activity Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B |
| NCBI Summary: | Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07476 |
| NCBI GenInfo Identifier: | 254763301 |
| NCBI Gene ID: | 3713 |
| NCBI Accession: | P07476.2 |
| UniProt Secondary Accession: | P07476,Q5T7P4, |
| UniProt Related Accession: | P07476 |
| Molecular Weight: | 68,479 Da |
| NCBI Full Name: | Involucrin |
| NCBI Synonym Full Names: | involucrin |
| NCBI Official Symbol: | IVL |
| NCBI Protein Information: | involucrin |
| UniProt Protein Name: | Involucrin |
| Protein Family: | Involucrin |
| UniProt Gene Name: | IVL |
| UniProt Entry Name: | INVO_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IVL antibody (CAB8026) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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