Anti-ITSN2 Antibody (CAB16525)
- SKU:
- CAB16525
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ITSN2 Antibody |
| Antibody SKU: | CAB16525 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, LO2, Jurkat, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GKHQ QISG RLQD VRLK KQTQ KTEL EVLD KQCD LEIM EIKQ LQQE LQEY QNKL IYLV PEKQ LLNE RIKN MQFS NTPD SGVS LLHK KSLE KEEL CQRL KEQL DALE KETA SKLS EMDS FNNQ L |
| Gene ID: | 50618 |
| Uniprot: | Q9NZM3 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 135kDa/141kDa/190kDa/193kDa |
| Observed MW: | 180kDa |
| Synonyms: | ITSN2, PRO2015, SH3D1B, SH3P18, SWA, SWAP |
| Background: | This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. |
| UniProt Protein Function: | Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes (PubMed:23999003). |
| NCBI Summary: | This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017] |
| UniProt Code: | Q9NZM3 |
| NCBI GenInfo Identifier: | 194294521 |
| NCBI Gene ID: | 50618 |
| NCBI Accession: | NP_006268.2 |
| UniProt Secondary Accession: | Q9NZM3,O95062, Q15812, Q9HAK4, Q9NXE6, Q9NYG0, Q9NZM2 Q9ULG4, |
| UniProt Related Accession: | Q9NZM3 |
| Molecular Weight: | 193 kDa |
| NCBI Full Name: | intersectin-2 isoform 1 |
| NCBI Synonym Full Names: | intersectin 2 |
| NCBI Official Symbol: | ITSN2 |
| NCBI Official Synonym Symbols: | SWA; SWAP; SH3D1B; SH3P18; PRO2015 |
| NCBI Protein Information: | intersectin-2 |
| UniProt Protein Name: | Intersectin-2 |
| UniProt Synonym Protein Names: | SH3 domain-containing protein 1B; SH3P18; SH3P18-like WASP-associated protein |
| Protein Family: | Intersectin |
| UniProt Gene Name: | ITSN2 |
 | Western blot analysis of extracts of various cell lines, using ITSN2 antibody (CAB16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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