Anti-IST1 Antibody (CAB9401)
- SKU:
- CAB9401
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-IST1 Antibody |
| Antibody SKU: | CAB9401 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human IST1 (NP_001257906.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HeLa, Mouse heart, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human IST1 (NP_001257906.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MLGS GFKA ERLR VNLR LVIN RLKL LEKK KTEL AQKA RKEI ADYL AAGK DERA RIRV EHII REDY LVEA MEIL ELYC DLLL ARFG LIQS MKEL DSGL AESV STLI WAAP RLQS EVAE LKIV ADQL CAKY SKEY GKLC RTNQ IGTV NDRL MHKL SVEA PPKI LVER YLIE IAKN YNVP YEPD SVVM AEAP PGVE TDLI DVGF TDDV KKGG PGRG GSGG FTAP VGGP DGTV PMPM PMPM PMPS ANTP FSYP LPKG PVDD INAD KNIS SAQI VGPG PKPE ASAK LPSR PADN YDNF VLPE LPSV PDTL PTAS AGAS TSAS EDID FDDL SRRF EELK KKT |
| Gene ID: | 9798 |
| Uniprot: | P53990 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Midbody, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 23kDa/36kDa/39kDa/41kDa |
| Observed MW: | 39kDa |
| Synonyms: | IST1, OLC1 |
| Background: | This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | IST1: Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Belongs to the IST1 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle; Cell cycle regulation Chromosomal Location of Human Ortholog: 16q22.2 Cellular Component: centrosome; cytoplasmic membrane-bound vesicle; ER-Golgi intermediate compartment; midbody; cytosol Molecular Function:protein domain specific binding; protein binding; protein complex binding Biological Process: release of virus from host; protein localization; establishment of protein localization; cell division; positive regulation of proteolysis; abscission; cytokinesis; viral capsid re-envelopment; positive regulation of collateral sprouting |
| NCBI Summary: | This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
| UniProt Code: | P53990 |
| NCBI GenInfo Identifier: | 41281489 |
| NCBI Gene ID: | 9798 |
| NCBI Accession: | NP_055576.2 |
| UniProt Related Accession: | P53990 |
| Molecular Weight: | 23kDa; 36kDa; 39kDa; 41kDa |
| NCBI Full Name: | IST1 homolog isoform b |
| NCBI Synonym Full Names: | IST1 factor associated with ESCRT-III |
| NCBI Official Symbol: | IST1 |
| NCBI Official Synonym Symbols: | OLC1; CHMP8 |
| NCBI Protein Information: | IST1 homolog |
| UniProt Protein Name: | IST1 homolog |
| UniProt Synonym Protein Names: | Putative MAPK-activating protein PM28 |
| Protein Family: | IST1 |
| UniProt Gene Name: | IST1 |
| UniProt Entry Name: | IST1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IST1 antibody (CAB9401) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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