Anti-ISG15 Antibody (CAB1182)

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SKU:
CAB1182
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:Anti-ISG15 Antibody
Antibody SKU:CAB1182
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, PC-3, SW620, HCT116
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human ISG15 (NP_005092.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGWD LTVK MLAG NEFQ VSLS SSMS VSEL KAQI TQKI GVHA FQQR LAVH PSGV ALQD RVPL ASQG LGPG STVL LVVD KCDE PLSI LVRN NKGR SSTY EVRL TQTV AHLK QQVS GLEG VQDD LFWL TFEG KPLE DQLP LGEY GLKP LSTV FMNL RLRG GGTE PGGR S
Gene ID:9636
Uniprot:P05161
Cellular Location:Cytoplasm, Secreted
Calculated MW:17kDa
Observed MW:14kDa
Synonyms:ISG15, G1P2, IFI15, IMD38, IP17, UCRP, hUCRP
Background:The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
UniProt Protein Function:ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
UniProt Protein Details:

Protein type:Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytosol; extracellular region; nucleoplasm

Molecular Function:protein binding

Biological Process: bypass DNA synthesis; defense response to bacterium; defense response to virus; ISG15-protein conjugation; negative regulation of interferon type I production; negative regulation of protein ubiquitination; negative regulation of viral genome replication; positive regulation of bone mineralization; regulation of interferon-gamma production

Disease: Immunodeficiency 38

NCBI Summary:The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012]
UniProt Code:P05161
NCBI GenInfo Identifier:52001470
NCBI Gene ID:9636
NCBI Accession:P05161.5
UniProt Secondary Accession:P05161,Q5SVA4, Q7Z2G2, Q96GF0,
UniProt Related Accession:P05161
Molecular Weight:17,888 Da
NCBI Full Name:Ubiquitin-like protein ISG15
NCBI Synonym Full Names:ISG15 ubiquitin-like modifier
NCBI Official Symbol:ISG15  
NCBI Official Synonym Symbols:G1P2; IP17; UCRP; IFI15; IMD38; hUCRP  
NCBI Protein Information:ubiquitin-like protein ISG15
UniProt Protein Name:Ubiquitin-like protein ISG15
UniProt Synonym Protein Names:Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; IP17; Ubiquitin cross-reactive protein; hUCRP
Protein Family:Ubiquitin-like protein
UniProt Gene Name:ISG15  
Anti-ISG15 Antibody (CAB1182)Western blot analysis of extracts of various cell lines, using ISG15 antibody (CAB1182) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-ISG15 Antibody (CAB1182)Immunohistochemistry of paraffin-embedded rat ovary using ISG15 antibody (CAB1182) at dilution of 1:100 (40x lens).
Anti-ISG15 Antibody (CAB1182)Immunohistochemistry of paraffin-embedded human thyroid cancer using ISG15 antibody (CAB1182) at dilution of 1:100 (40x lens).

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