Anti-IPO4 Antibody (CAB15600)
- SKU:
- CAB15600
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-IPO4 Antibody |
| Antibody SKU: | CAB15600 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 728-1081 of human IPO4 (NP_078934.3). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SH-SY5Y, A-549, HepG2, Mouse brain, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 728-1081 of human IPO4 (NP_078934.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LHKA CQSC PSEP NTAA LQAA LARV VPSY MQAV NRER ERQV VMAV LEAL TGVL RSCG TLTL KPPG RLAE LCGV LKAV LQRK TACQ DTDE EEEE EDDD QAEY DAML LEHA GEAI PALA AAAG GDSF APFF AGFL PLLV CKTK QGCT VAEK SFAV GTLA ETIQ GLGA ASAQ FVSR LLPV LLST AQEA DPEV RSNA IFGM GVLA EHGG HPAQ EHFP KLLG LLFP LLAR ERHD RVRD NICG ALAR LLMA SPTR KPEP QVLA ALLH ALPL KEDL EEWV TIGR LFSF LYQS SPDQ VIDV APEL LRIC SLIL ADNK IPPD TKAA LLLL LTFL AKQH TDSF QAAL GSLP VDKA QELQ AVLG LS |
| Gene ID: | 79711 |
| Uniprot: | Q8TEX9 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 119kDa |
| Observed MW: | 119kDa |
| Synonyms: | IPO4, Imp4 |
| Background: |
| UniProt Protein Function: | IPO4: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear import; Karyopherin Chromosomal Location of Human Ortholog: 14q12 Cellular Component: protein complex; membrane; nuclear chromatin; cytoplasm; nuclear pore Molecular Function:protein binding; Ran GTPase binding Biological Process: intracellular protein transport; protein transport; DNA replication-independent nucleosome assembly; DNA replication-dependent nucleosome assembly |
| UniProt Code: | Q8TEX9 |
| NCBI GenInfo Identifier: | 62460637 |
| NCBI Gene ID: | 79711 |
| NCBI Accession: | NP_078934.3 |
| UniProt Secondary Accession: | Q8TEX9,Q2NL96, Q86TZ9, Q8NCG8, Q96SJ3, Q9BTI4, Q9H5L0 B2RN95, |
| UniProt Related Accession: | Q8TEX9 |
| Molecular Weight: | 118,715 Da |
| NCBI Full Name: | importin-4 |
| NCBI Synonym Full Names: | importin 4 |
| NCBI Official Symbol: | IPO4 |
| NCBI Official Synonym Symbols: | Imp4 |
| NCBI Protein Information: | importin-4; imp4b; ranBP4; importin-4b; ran-binding protein 4 |
| UniProt Protein Name: | Importin-4 |
| UniProt Synonym Protein Names: | Importin-4b; Imp4b; Ran-binding protein 4; RanBP4 |
| Protein Family: | Importin |
| UniProt Gene Name: | IPO4 |
| UniProt Entry Name: | IPO4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IPO4 antibody (CAB15600) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of C6 cells using IPO4 antibody (CAB15600) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using IPO4 antibody (CAB15600) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using IPO4 antibody (CAB15600) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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