Anti-INSIG1 Antibody (CAB16278)
- SKU:
- CAB16278
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-INSIG1 Antibody |
| Antibody SKU: | CAB16278 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human INSIG1 (NP_005533.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human INSIG1 (NP_005533.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LWWT FDRS RSGL GLGI TIAF LATL ITQF LVYN GVYQ YTSP DFLY IRSW LPCI FFSG GVTV GNIG RQLA MGVP EKPH SD |
| Gene ID: | 3638 |
| Uniprot: | O15503 |
| Cellular Location: | |
| Calculated MW: | 17kDa/29kDa |
| Observed MW: | 36kDa |
| Synonyms: | INSIG1, CL6 |
| Background: | This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | INSIG1: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Belongs to the INSIG family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 7q36 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane Molecular Function:protein binding Biological Process: cell proliferation; metabolic process; SREBP-mediated signaling pathway |
| NCBI Summary: | Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2009] |
| UniProt Code: | O15503 |
| NCBI GenInfo Identifier: | 115502405 |
| NCBI Gene ID: | 3638 |
| NCBI Accession: | O15503.3 |
| UniProt Secondary Accession: | O15503,Q53XW8, Q9BUV5, A4D2N1, A8K6L0, |
| UniProt Related Accession: | O15503 |
| Molecular Weight: | 17,530 Da |
| NCBI Full Name: | Insulin-induced gene 1 protein |
| NCBI Synonym Full Names: | insulin induced gene 1 |
| NCBI Official Symbol: | INSIG1 |
| NCBI Official Synonym Symbols: | CL6; CL-6 |
| NCBI Protein Information: | insulin-induced gene 1 protein |
| UniProt Protein Name: | Insulin-induced gene 1 protein |
| Protein Family: | Insulin-induced gene 1 protein |
| UniProt Gene Name: | INSIG1 |
| UniProt Entry Name: | INSI1_HUMAN |
 | Western blot analysis of extracts of rat brain, using INSIG1 antibody (CAB16278) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 150s. |
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