Anti-Infliximab (Remicade®) ELISA Kit
- SKU:
- HUMB00002
- Product Type:
- ELISA Kit
- ELISA Type:
- Biosimilar ELISA
- Biosimilar ELISA Type:
- Qualitative
- Applications:
- ELISA
- Reactivity:
- Human
- Analytes:
- Infliximab (Remicade®)
- Research Area:
- Anti-TNF Alpha
Description
Anti-Infliximab (Remicade®) Qualitative ELISA Kit
Enzyme-linked immunosorbent assay for the qualitative determination of specific antibodies to Infliximab in human serum and plasma with confirmation. Infliximab (Remicade) is associated with the development of anti-Infliximab antibodies. The Assay Genie Antibody to Infliximab ELISA Kit can be efficiently used for the determination of anti-drug antibody (ADA) monitoring of antibodies to Infliximab,
Anti-Infliximab (Remicade®) Qualitative ELISA Kit test principle
The Assay Genie Antibody to infliximab (Remicade®) ELISA is a sandwich assay for the determination of antibodies against infliximab in serum and plasma samples. During the first incubation period, antibodies to infliximab (ATI) in patient serum/ plasma samples are captured by the drug infliximab (Remicade®) coated on the wall of the microtiter wells. After washing away the unbound components from samples, a peroxidase-labelled specific conjugate is added to each well and then incubated. After a second washing step, the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The intensity of the reaction color is directly proportional to the concentration of ATI in sample.
Anti-Infliximab Qualitative ELISA Product Information
| Information | Description |
Application | Free drug |
Required Volume (uL) | 10 |
Total Time (min) | 140 |
Sample Type | Serum, Plasma |
Number of Assays | 96 |
Detection Limit (ng/mL) | 15 (ng/mL) |
Spike Recovery (%) | 85-115% |
Shelf Life (year) | 1 |
Alternative Names | Human Tumour Necrosis Factor Alpha |
Anti-Infliximab Qualitative ELISA - Key Information
Infliximab mode of action
Infliximab (Remicade®) is a chimeric monoclonal antibody and used to treat autoimmune disorders. Infliximab reduces the amount of active human tumour necrosis factor alpha (hTNF alpha) in the body by binding to it and preventing it from signalling the receptors for TNF alpha on the surface of various cell types. TNF alpha is one of the key cytokines that triggers and sustains the inflammatory reactions.
Infliximab treatment
Infliximab is used for the treatment of psoriasis, Crohn’s disease, ankylosing spondylitis, psoriatic arthritis, rheumatoid arthritis, ulcerative colitis, and is approved by FDA.
Infliximab Immunogenicity
One of the major concern, despite of its wide usage, is potential development of anti-infliximab antibodies which in turn may interfere with infliximab (Remicade®) efficacy as mainly judged by observing the relapse of signs and symptoms of disease and necessitate dose-escalation or potentially ending up the treatment.
In this context, demonstration of anti-infliximab antibodies during treatment with infliximab (Remicade®) has a major concern and monitoring for the presence and/or quantitation of specific antibodies during clinical trials is an important issue for follow up of the treatment regimens. The Assay Genie anti-infliximab antibody ELISA Kit can be efficiently used for monitoring infliximab-specific antibodies (anti-drug antibbodies) and is for research use only.
Anti-Infliximab Qualitative ELISA Kit Contents
| Size | Kit contents |
1 x 12 x 8 | Microtiter Plate Break apart strips. Microtiter plate with 12 rows each of 8 wells coated with infliximab. |
1 x 0.25 | Reactive Control |
1 x 0.5 mL | Negative Control |
1 x 12 mL | Assay Buffer |
1 x 12 mL | Peroxidase Conjugate |
1 x 12 mL | TMB Substrate Solution |
1 x 12 mL | TMB Stop Solution |
1 x 50mL | Wash Buffer, concentrate (20x) Contains Buffer with Tween 20. |
2 x 1 | Adhesive Film |
Anti-Infliximab Qualitative ELISA Kit Protocol
| Steps | Protocol |
1 | Pipette 100µl of Assay Buffer non-exceptionally into each of the wells to be used. |
2 | QUALITATIVE ELISA TEST FORMAT |
3 | Cover the plate with adhesive film. Briefly mix contents by gently shaking the plate. Incubate 60 min at room temperature (18-25°C). |
4 | Remove adhesive film. Discard incubation solution. Wash plate 3 times each with 300 µL of diluted Wash Buffer. Remove excess solution by tapping the inverted plate on a paper towel. |
5 | Pipette 100 µL of ready-to use Peroxidase Conjugate into each well. |
6 | Cover the plate with adhesive film. Incubate 60 min at room temperature (18- 25°C). |
7 | Remove adhesive film. Discard incubation solution. Wash plate 3 times each with 300 µL of diluted Wash Buffer. Remove excess solution by tapping the inverted plate on a paper towel. |
8 | Pipette 100 µL of TMB Substrate Solution into each well. |
9 | Incubate 20 min (without adhesive foil) at room temperature (18-25°C) in the dark. |
10 | Stop the substrate reaction by adding 100 µL of Stop Solution into each well. Briefly mix contents by gently shaking the plate. Colour changes from blue to yellow. |
11 | Measure optical density with a photometer at 450/650 nm within 30 min after pipetting of the Stop Solution. |
Trademarks
Remicade® is a registered trademark of Janssen Biotech Inc.
