Anti-INCENP Antibody (CAB0622)
- SKU:
- CAB0622
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-INCENP Antibody |
| Antibody SKU: | CAB0622 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 785-914 of human INCENP (NP_064623.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | HeLa, Jurkat, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 785-914 of human INCENP (NP_064623.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ALNV TVDV QSPA CTSY QMTP QGHR APPK INPD NYGM DLNS DDST DDEA HPRK PIPT WARG TPLS QAII HQYY HPPN LLEL FGTI LPLD LEDI FKKS KPRY HKRT SSAV WNSP PLQG ARVP SSLA YSLK KH |
| Gene ID: | 3619 |
| Uniprot: | Q9NQS7 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore, spindle |
| Calculated MW: | 104kDa/105kDa |
| Observed MW: | 120-140kDa |
| Synonyms: | INCENP |
| Background: |
| NCBI Summary: | In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NQS7 |
| NCBI GenInfo Identifier: | 102467235 |
| NCBI Gene ID: | 3619 |
| NCBI Accession: | NP_001035784.1 |
| UniProt Related Accession: | Q9NQS7 |
| Molecular Weight: | |
| NCBI Full Name: | inner centromere protein isoform 1 |
| NCBI Synonym Full Names: | inner centromere protein |
| NCBI Official Symbol: | INCENP |
| NCBI Protein Information: | inner centromere protein |
| UniProt Protein Name: | Inner centromere protein |
| Protein Family: | Inclusion membrane protein |
| UniProt Gene Name: | INCENP |
| UniProt Entry Name: | INCE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using INCENP antibody (CAB0622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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