Anti-InaD-like protein Antibody (CAB8476)
- SKU:
- CAB8476
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-InaD-like protein Antibody |
| Antibody SKU: | CAB8476 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-680 of human INADL (NP_795352.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SKOV3, SW480, 293T, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 450-680 of human INADL (NP_795352.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VRRK TSSS TSPL EPPS DRGT VVEP LKPP ALFL TGAV ETET NVDG EDEE IKER IDTL KNDN IQAL EKLE KVPD SPEN ELKS RWEN LLGP DYEV MVAT LDTQ IADD AELQ KYSK LLPI HTLR LGVE VDSF DGHH YISS IVSG GPVD TLGL LQPE DELL EVNG MQLY GKSR REAV SFLK EVPP PFTL VCCR RLFD DEAS VDEP RRTE TSLP ETEV DHNM DVNT EEDD DGEL ALW |
| Gene ID: | 10207 |
| Uniprot: | Q8NI35 |
| Cellular Location: | Apical cell membrane, Cell junction, Cell membrane, Cytoplasm, Peripheral membrane protein, perinuclear region, tight junction |
| Calculated MW: | 125kDa/167kDa/170kDa/173kDa/196kDa |
| Observed MW: | 255kDa |
| Synonyms: | PATJ, Cipp, INADL, InaD-like, hINADL |
| Background: | This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. |
| UniProt Protein Function: | Scaffolding protein that may bring different proteins into adjacent positions at the cell membrane. May regulate protein targeting, cell polarity and integrity of tight junctions. May regulate the surface expression and/or function of ASIC3 in sensory neurons. May recruit ARHGEF18 to apical cell-cell boundaries (PubMed:22006950). |
| NCBI Summary: | This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8NI35 |
| NCBI GenInfo Identifier: | 116242542 |
| NCBI Gene ID: | 10207 |
| NCBI Accession: | Q8NI35.3 |
| UniProt Secondary Accession: | Q8NI35,O15249, O43742, O60833, Q5VUA5, Q5VUA6, Q5VUA7 Q5VUA8, Q5VUA9, Q5VUB0, Q8WU78, Q9H3N9, |
| UniProt Related Accession: | Q8NI35 |
| Molecular Weight: | 125,231 Da |
| NCBI Full Name: | InaD-like protein |
| NCBI Synonym Full Names: | PATJ, crumbs cell polarity complex component |
| NCBI Official Symbol: | PATJ |
| NCBI Official Synonym Symbols: | Cipp; INADL; hINADL; InaD-like |
| NCBI Protein Information: | inaD-like protein |
| UniProt Protein Name: | InaD-like protein |
| UniProt Synonym Protein Names: | Pals1-associated tight junction protein; Protein associated to tight junctions |
| Protein Family: | Patj |
| UniProt Gene Name: | PATJ |
 | Western blot analysis of extracts of various cell lines, using INADL antibody (CAB8476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using INADL antibody (CAB8476) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using INADL antibody (CAB8476) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using INADL antibody (CAB8476) at dilution of 1:100 (40x lens). |
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