Anti-ILK Antibody (CAB0901)
- SKU:
- CAB0901
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ILK Antibody |
| Antibody SKU: | CAB0901 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HeLa, Mouse kidney, 293T, NIH/3T3, MCF7, A-549, Mouse heart, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDDI FTQC REGN AVAV RLWL DNTE NDLN QGDD HGFS PLHW ACRE GRSA VVEM LIMR GARI NVMN RGDD TPLH LAAS HGHR DIVQ KLLQ YKAD INAV NEHG NVPL HYAC FWGQ DQVA EDLV ANGA LVSI CNKY GEMP VDKA KAPL RELL RERA EKMG QNLN RIPY KDTF WKGT TRTR PRNG TLNK HSGI DFKQ LNFL TKLN ENHS GELW KGRW QGND IVVK VLKV RDWS TRKS RDFN EECP RLRI FSHP NVLP VLGA CQSP |
| Gene ID: | 3611 |
| Uniprot: | Q13418 |
| Cellular Location: | Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, focal adhesion, lamellipodium, myofibril, sarcomere |
| Calculated MW: | 36kDa/44kDa/51kDa |
| Observed MW: | 48kDa |
| Synonyms: | ILK, HEL-S-28, ILK-1, ILK-2, P59, p59ILK |
| Background: | This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; MLK family; ILK subfamily Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: cell junction; cytoplasm; cytosol; focal adhesion; membrane; nucleoplasm Molecular Function:protein binding; protein kinase binding; protein serine/threonine kinase activity Biological Process: cell-matrix adhesion; integrin-mediated signaling pathway; positive regulation of phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q13418 |
| NCBI GenInfo Identifier: | 9973397 |
| NCBI Gene ID: | 3611 |
| NCBI Accession: | Q13418.2 |
| UniProt Secondary Accession: | Q13418,P57043, Q68DZ3, B7Z1I0, B7Z418, D3DQU0, |
| UniProt Related Accession: | Q13418 |
| Molecular Weight: | 36,467 Da |
| NCBI Full Name: | Integrin-linked protein kinase |
| NCBI Synonym Full Names: | integrin linked kinase |
| NCBI Official Symbol: | ILK |
| NCBI Official Synonym Symbols: | P59; ILK-1; ILK-2; p59ILK; HEL-S-28 |
| NCBI Protein Information: | integrin-linked protein kinase |
| UniProt Protein Name: | Integrin-linked protein kinase |
| UniProt Synonym Protein Names: | 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK |
| Protein Family: | Integrin-linked protein kinase |
| UniProt Gene Name: | ILK |
| UniProt Entry Name: | ILK_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ILK antibody (CAB0901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody (CAB0901) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody (CAB0901) at dilution of 1:200 (40x lens). |
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