Anti-IL-6ST Antibody (CAB18036)[KO Validated]

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SKU:
CAB18036
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:[KO Validated] IL6ST Rabbit Polyclonal Antibody
Antibody SKU:CAB18036
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 678-857 of human IL6ST (NP_001177910.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 678-857 of human IL6ST (NP_001177910.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSIS SSDE NESS QNTS STVQ YSTV VHSG YRHQ VPSV QVFS RSES TQPL LDSE ERPE DLQL VDHV DGGD GILP RQQY FKQN CSQH ESSP DISH FERS KQVS SVNE EDFV RLKQ QISD HISQ SCGS GQMK MFQE VSAA DAFG PGTE GQVE RFET VGME AATD EGMP KSYL PQTV RQGG YMPQ
Gene ID:3572
Uniprot:P40189
Cellular Location:Cell membrane, Secreted, Single-pass type I membrane protein
Calculated MW:103kDa
Observed MW:150KDa
Anti-IL-6ST Antibody [KO Validated] (CAB18036)Western blot analysis of extracts from normal (control) and IL6ST knockout (KO) A-549 cells, using IL6ST antibody (CAB18036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Anti-IL-6ST Antibody [KO Validated] (CAB18036)Western blot analysis of extracts of various cell lines, using IL6ST antibody (CAB18036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:IL6ST, CD130, CDW130, GP130, IL-6RB
Background:The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17.
UniProt Protein Function:gp130: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region; interleukin-6 receptor complex; membrane; oncostatin-M receptor complex; plasma membrane

Molecular Function:ciliary neurotrophic factor receptor activity; ciliary neurotrophic factor receptor binding; growth factor binding; interleukin-27 receptor activity; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor binding; leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; protein binding; protein homodimerization activity

Biological Process: cytokine and chemokine mediated signaling pathway; leukemia inhibitory factor signaling pathway; negative regulation of apoptosis; positive regulation of acute inflammatory response; positive regulation of adaptive immune response; positive regulation of cell proliferation; positive regulation of osteoblast differentiation; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tyrosine phosphorylation of Stat3 protein; response to cytokine stimulus

NCBI Summary:The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
UniProt Code:P40189
NCBI GenInfo Identifier:215273999
NCBI Gene ID:3572
NCBI Accession:P40189.2
UniProt Secondary Accession:P40189,Q5FC04, Q9UQ41, A0N0L4,
UniProt Related Accession:P40189
Molecular Weight:96,276 Da
NCBI Full Name:Interleukin-6 receptor subunit beta
NCBI Synonym Full Names:interleukin 6 signal transducer
NCBI Official Symbol:IL6ST  
NCBI Official Synonym Symbols:CD130; GP130; CDW130; IL-6RB  
NCBI Protein Information:interleukin-6 receptor subunit beta
UniProt Protein Name:Interleukin-6 receptor subunit beta
UniProt Synonym Protein Names:CDw130; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD_antigen: CD130
Protein Family:Interleukin-6 receptor
UniProt Gene Name:IL6ST  
UniProt Entry Name:IL6RB_HUMAN

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