Anti-IL-1RN Antibody (CAB2088)
- SKU:
- CAB2088
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | IL1RN Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB2088 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of human IL1RN (NP_000568.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, NIH/3T3, HL-60, BT-474, HT-29 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-159 of human IL1RN (NP_000568.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MALE TICR PSGR KSSK MQAF RIWD VNQK TFYL RNNQ LVAG YLQG PNVN LEEK IDVV PIEP HALF LGIH GGKM CLSC VKSG DETR LQLE AVNI TDLS ENRK QDKR FAFI RSDS GPTT SFES AACP GWFL CTAM EADQ PVSL TNMP DEGV MVTK FYFQ EDE |
| Gene ID: | 3557 |
| Uniprot: | P18510 |
| Cellular Location: | Cytoplasm, Secreted |
| Calculated MW: | 16kDa/17kDa/19kDa/20kDa |
| Observed MW: | 23kDa |
| Synonyms: | IL1RN, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 |
| Background: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| UniProt Protein Function: | IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Vesicle; Cytokine; Secreted Chromosomal Location of Human Ortholog: 2q14.2 Cellular Component: extracellular space; cytoplasm; plasma membrane; intracellular Molecular Function:interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity Biological Process: response to drug; positive regulation of JNK activity; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; response to glucocorticoid stimulus; carboxylic acid metabolic process; response to lipopolysaccharide; female pregnancy; sensory perception of pain; fever; chronic inflammatory response to antigenic stimulus; memory; response to starvation; insulin secretion; acute-phase response; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to activity; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis Disease: Microvascular Complications Of Diabetes, Susceptibility To, 4; Gastric Cancer, Hereditary Diffuse; Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis |
| NCBI Summary: | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18510 |
| NCBI GenInfo Identifier: | 124312 |
| NCBI Gene ID: | 3557 |
| NCBI Accession: | P18510.1 |
| UniProt Secondary Accession: | P18510,Q14628, Q53SC2, Q7RTZ4, Q96GD6, Q9UPC0, A8K4G1 |
| UniProt Related Accession: | P18510 |
| Molecular Weight: | 16,142 Da |
| NCBI Full Name: | Interleukin-1 receptor antagonist protein |
| NCBI Synonym Full Names: | interleukin 1 receptor antagonist |
| NCBI Official Symbol: | IL1RN |
| NCBI Official Synonym Symbols: | DIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA |
| NCBI Protein Information: | interleukin-1 receptor antagonist protein; IL1 inhibitor; IL1RN (IL1F3); type II interleukin-1 receptor antagonist; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra) |
| UniProt Protein Name: | Interleukin-1 receptor antagonist protein |
| UniProt Synonym Protein Names: | ICIL-1RA; IL1 inhibitor; INN: Anakinra |
| Protein Family: | Interleukin-1 receptor antagonist protein |
| UniProt Gene Name: | IL1RN |
| UniProt Entry Name: | IL1RA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL1RN antibody (CAB2088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of human skin cancer using IL1RN Rabbit pAb (CAB2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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