Anti-IL-18 Antibody (CAB1115)[KO Validated]

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SKU:
CAB1115
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Frequently bought together:

Description

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Antibody Name:[KO Validated] IL18 Rabbit Polyclonal Antibody
Antibody SKU:CAB1115
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YFGK LESK LSVI RNLN DQVL FIDQ GNRP LFED MTDS DCRD NAPR TIFI ISMY KDSQ PRGM AVTI SVKC EKIS TLSC ENKI ISFK EMNP PDNI KDTK SDII FFQR SVPG HDNK MQFE SSSY EGYF LACE KERD LFKL ILKK EDEL GDRS IMFT VQNE D
Gene ID:3606
Uniprot:Q14116
Cellular Location:Secreted
Calculated MW:21kDa/22kDa
Observed MW:23kDa
Synonyms:IL18, IGIF, IL-18, IL-1g, IL1F4
Background:The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.
UniProt Protein Details:

Protein type:Cytokine

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function:protein binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of NK T cell proliferation; natural killer cell activation; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; chemokine biosynthetic process; cell-cell signaling; lipopolysaccharide-mediated signaling pathway; granulocyte macrophage colony-stimulating factor biosynthetic process; angiogenesis; inflammatory response; positive regulation of interleukin-17 production; regulation of cell adhesion; positive regulation of natural killer cell proliferation; T-helper 1 type immune response; T-helper 2 type immune response; interleukin-2 biosynthetic process; MAPKKK cascade; interleukin-13 biosynthetic process; sleep; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; interferon-gamma biosynthetic process; immune response; negative regulation of myoblast differentiation; positive regulation of inflammatory response

NCBI Summary:The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Code:Q14116
NCBI GenInfo Identifier:3219817
NCBI Gene ID:3606
NCBI Accession:Q14116.1
UniProt Secondary Accession:Q14116,O75599, Q6FGY3, Q6WWJ7,
UniProt Related Accession:Q14116
Molecular Weight:21,896 Da
NCBI Full Name:Interleukin-18
NCBI Synonym Full Names:interleukin 18
NCBI Official Symbol:IL18  
NCBI Official Synonym Symbols:IGIF; IL-18; IL-1g; IL1F4  
NCBI Protein Information:interleukin-18; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor)
UniProt Protein Name:Interleukin-18
UniProt Synonym Protein Names:Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma
Protein Family:Interleukin
UniProt Gene Name:IL18  
UniProt Entry Name:IL18_HUMAN
Anti-IL-18 Antibody [KO Validated] (CAB1115)Western blot analysis of extracts of various cell lines, using IL18 antibody (CAB1115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-IL-18 Antibody [KO Validated] (CAB1115)Western blot analysis of extracts from normal (control) and IL18 knockout (KO) HeLa cells, using IL18 antibody (CAB1115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-IL-18 Antibody [KO Validated] (CAB1115)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IL18 antibody (CAB1115). Western blot was performed from the immunoprecipitate using IL18 antibody (CAB1115) at a dilition of 1:1000.

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