Anti-IL-10 Antibody (CAB2171)
- SKU:
- CAB2171
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | IL10 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB2171 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-178 of human IL10 (NP_000563.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SPGQ GTQS ENSC THFP GNLP NMLR DLRD AFSR VKTF FQMK DQLD NLLL KESL LEDF KGYL GCQA LSEM IQFY LEEV MPQA ENQD PDIK AHVN SLGE NLKT LRLR LRRC HRFL PCEN KSKA VEQV KNAF NKLQ EKGI YKAM SEFD IFIN YIEA YMTM KIRN |
| Gene ID: | 3586 |
| Uniprot: | P22301 |
| Cellular Location: | Secreted |
| Calculated MW: | 20kDa |
| Observed MW: | 22kDa |
| Synonyms: | CSIF, GVHDS, IL-10, IL10A, TGIF, IL10 |
| Background: | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| UniProt Protein Function: | IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 1q31-q32 Cellular Component: extracellular space Molecular Function:growth factor activity; cytokine activity; interleukin-10 receptor binding Biological Process: B cell proliferation; negative regulation of chronic inflammatory response to antigenic stimulus; regulation of isotype switching; positive regulation of transcription, DNA-dependent; leukocyte chemotaxis; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; response to insulin stimulus; negative regulation of interferon-alpha biosynthetic process; negative regulation of T cell proliferation; cytoplasmic sequestering of NF-kappaB; cell-cell signaling; positive regulation of MHC class II biosynthetic process; negative regulation of interferon-gamma production; response to molecule of bacterial origin; negative regulation of interleukin-6 production; hemopoiesis; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; aging; response to drug; response to inactivity; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; T-helper 2 type immune response; negative regulation of myeloid dendritic cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of cytokine secretion during immune response; regulation of gene expression; B cell differentiation; defense response to bacterium; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; receptor biosynthetic process; response to activity; regulation of sensory perception of pain; negative regulation of apoptosis; positive regulation of cytokine secretion Disease: Graft-versus-host Disease, Susceptibility To; Rheumatoid Arthritis; Human Immunodeficiency Virus Type 1, Susceptibility To |
| NCBI Summary: | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011] |
| UniProt Code: | P22301 |
| NCBI GenInfo Identifier: | 124292 |
| NCBI Gene ID: | 3586 |
| NCBI Accession: | P22301.1 |
| UniProt Related Accession: | P22301 |
| Molecular Weight: | 20,517 Da |
| NCBI Full Name: | Interleukin-10 |
| NCBI Synonym Full Names: | interleukin 10 |
| NCBI Official Symbol: | IL10 |
| NCBI Official Synonym Symbols: | CSIF; TGIF; GVHDS; IL-10; IL10A |
| NCBI Protein Information: | interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor |
| UniProt Protein Name: | Interleukin-10 |
| UniProt Synonym Protein Names: | Cytokine synthesis inhibitory factor; CSIF |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL10 |
| UniProt Entry Name: | IL10_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using IL10 antibody (CAB2171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using IL10 antibody (CAB2171) at dilution of 1:200 (40x lens). |
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